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Team:Yale/Project Export
From 2013.igem.org
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== Aims for the Project == | == Aims for the Project == | ||
| - | #'''Engineer strains of ''E. coli'' | + | #'''Engineer strains of ''E. coli'' for PLA synthesis'''<br> |
#'''Develop bioassay to screen PLA production'''<br> | #'''Develop bioassay to screen PLA production'''<br> | ||
#'''Apply MAGE to optimize PLA production, guided by FBA'''<br> | #'''Apply MAGE to optimize PLA production, guided by FBA'''<br> | ||
Revision as of 02:20, 6 September 2013
| Project Overview | Validate PLA synthesis | Develop bioassay | Apply MAGE | Introduce export system | Make a bioplastic |
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Aims for the Project
- Engineer strains of E. coli for PLA synthesis
- Develop bioassay to screen PLA production
- Apply MAGE to optimize PLA production, guided by FBA
- Introduce type 1 secretion system to export and extract PLA
- Make a bioplastic
Introduce type 1 secretion system to export and extract PLA
- We needed a way to export the PLA once it was synthesized by the E. coli
- We found the paper by Linton 2010 where she focused on exporting PHA from engineered E. coli
- She used Phasin, a PHA granule associated protein that plays a role in granule formation, with a hlyA tag.
- This allowed the cells to export the PLA since the hlyA tag was attached to the granule
- Due to the similarity between PHA granules and PLA granules we hypothesized that this same export system would allow us to export PLA from our cells.
