Team:Groningen/Labwork/6 September 2013

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<br><img src="https://static.igem.org/mediawiki/2013/1/1f/ColonyPCR_pSB1C3-S2-S5.jpg" width="50%" >
<br><img src="https://static.igem.org/mediawiki/2013/1/1f/ColonyPCR_pSB1C3-S2-S5.jpg" width="50%" >
<br>pSB1C3-S2-S5 colonies.
<br>pSB1C3-S2-S5 colonies.
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<br>Two colonies seem to be positive candidates.
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<br>Colony A from the plate pSB1C3-S1-S5 was inoculated over-night in liquid culture (LB + Cm).
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<br>Colony B from the plate pSB1C3-S2-S5 was inoculated over-night in liquid culture (LB + Cm).
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Revision as of 10:41, 6 September 2013

Claudio

The plates (pSB1C3-S1-S5 and pSB1C3-S2-S5) show single colonies.
ColonyPCR was performed on 6 colonies per plate (total of 12 colonies) beside one positive control (pSB1C3-S1).
The primers used were VF2 and VR (annealing temperature 56°C).
The samples were checked on agarose gel 0.8%.

pSB1C3-S1-S5 colonies.


pSB1C3-S2-S5 colonies.

Two colonies seem to be positive candidates.
Colony A from the plate pSB1C3-S1-S5 was inoculated over-night in liquid culture (LB + Cm).
Colony B from the plate pSB1C3-S2-S5 was inoculated over-night in liquid culture (LB + Cm).