Team:Groningen/Labwork/6 September 2013

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<br>ColonyPCR was performed on 6 colonies per plate (total of 12 colonies) beside one positive control (pSB1C3-S1). <br>The primers used were VF2 and VR (annealing temperature 56&deg;C).
<br>ColonyPCR was performed on 6 colonies per plate (total of 12 colonies) beside one positive control (pSB1C3-S1). <br>The primers used were VF2 and VR (annealing temperature 56&deg;C).
<br>The samples were checked on agarose gel 0.8%.
<br>The samples were checked on agarose gel 0.8%.
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<br><img src="https://static.igem.org/mediawiki/2013/6/69/ColonyPCR_pSB1C3-S1-S5.jpg" width="50%" >
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<br>pSB1C3-S1-S5 colonies.
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<br>
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<br><img src="https://static.igem.org/mediawiki/2013/1/1f/ColonyPCR_pSB1C3-S2-S5.jpg" width="50%" >
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<br>pSB1C3-S2-S5 colonies.
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<br>
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<br>Two colonies seem to be positive candidates.
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<br>Colony A from the plate pSB1C3-S1-S5 was inoculated over-night in liquid culture (LB + Cm).
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<br>Colony C from the plate pSB1C3-S2-S5 was inoculated over-night in liquid culture (LB + Cm).
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<h2>Sebas</h2>
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<br>All inoculations form yesterday grew.
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<br>After spinning down for plasmid isolation, some pellets were green/red (indicating that the promoter is inserted right)
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<br>
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<img src="https://static.igem.org/mediawiki/2013/c/ce/BB_GFP_and_pXs1s13.jpg" width="50%"></img>
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<Br>Row1: GFP0804 (1-4) pXs1s13 (5-8)
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<br>Row2: P:RFP  (1-4) GFPdsm  (5-8)
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<Br>
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<Br>
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<Br>
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Did an restriction check on pXs1s13 (silk) with EcoRI, analysis of the gel showed that the silk did not ligate into the pX plasmid.
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</div>
</div>

Latest revision as of 10:52, 6 September 2013

Claudio

The plates (pSB1C3-S1-S5 and pSB1C3-S2-S5) show single colonies.
ColonyPCR was performed on 6 colonies per plate (total of 12 colonies) beside one positive control (pSB1C3-S1).
The primers used were VF2 and VR (annealing temperature 56°C).
The samples were checked on agarose gel 0.8%.

pSB1C3-S1-S5 colonies.


pSB1C3-S2-S5 colonies.

Two colonies seem to be positive candidates.
Colony A from the plate pSB1C3-S1-S5 was inoculated over-night in liquid culture (LB + Cm).
Colony C from the plate pSB1C3-S2-S5 was inoculated over-night in liquid culture (LB + Cm).

Sebas


All inoculations form yesterday grew.
After spinning down for plasmid isolation, some pellets were green/red (indicating that the promoter is inserted right)

Row1: GFP0804 (1-4) pXs1s13 (5-8)
Row2: P:RFP (1-4) GFPdsm (5-8)


Did an restriction check on pXs1s13 (silk) with EcoRI, analysis of the gel showed that the silk did not ligate into the pX plasmid.