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Team:Washington/DNA SEQUENCING
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<p>Create a COPY of the <a href="https://docs.google.com/spreadsheet/ccc?key=0Alg6O8T70xv1dFpQMHdFYVBPeEliUXd5MU9WSGQ0RWc&usp=sharing">genewiz_premix</a> document (in folder) and rename it with today’s date. </p> | <p>Create a COPY of the <a href="https://docs.google.com/spreadsheet/ccc?key=0Alg6O8T70xv1dFpQMHdFYVBPeEliUXd5MU9WSGQ0RWc&usp=sharing">genewiz_premix</a> document (in folder) and rename it with today’s date. </p> | ||
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<li>Enter your DNA Name (make sure it is not redundant and in order of wells you are submitting) </li> | <li>Enter your DNA Name (make sure it is not redundant and in order of wells you are submitting) </li> | ||
<li>DNA Type --> "Plasmid" (click the Type button to autofill the rest, same for all others) </li> | <li>DNA Type --> "Plasmid" (click the Type button to autofill the rest, same for all others) </li> | ||
Revision as of 07:03, 7 September 2013
DNA Sequencing
- Add 10uL of miniprep DNA (at least 50ng/ul) + 5 uL of primer to a PCR Strip Tube. The only labels on the Tube should be AC01 ... ACXX (depending on the number of samples, XX)
Create a COPY of the genewiz_premix document (in folder) and rename it with today’s date.
- Enter your DNA Name (make sure it is not redundant and in order of wells you are submitting)
- DNA Type --> "Plasmid" (click the Type button to autofill the rest, same for all others)
- DNA Length --> "6000kb"
- Primer Name → i.e. "VF2" or "VR" (if you want forward or reverse, respectively)
Send Google doc to Advisor in sharing options.
- Print out receipt (Send to an advisor and we can print it out) and place labeled samples (AC01 ... ACXX) and order sheet in a Plastic Bag and then Drop Box
- When sequencing is completed you can view the results by logging in going to the appropriate link under "Recent Orders" on the lower right side of the screen
