Team:Washington/DNA SEQUENCING

From 2013.igem.org

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<li><a href="https://2013.igem.org/Team:Washington">Home</a>
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<ul>
 +
<li><a href="https://2013.igem.org/Team:Washington">UW 2013</a></li>
 +
<li><a href="https://2012.igem.org/Team:Washington">UW 2012</a></li>
 +
<li><a href="https://2011.igem.org/Team:Washington">UW 2011</a></li>
 +
<li><a href="https://2010.igem.org/Team:Washington">UW 2010</a></li>
 +
<li><a href="https://2009.igem.org/Team:Washington">UW 2009</a></li>
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<li><a href="https://2008.igem.org/Team:University_of_Washington">UW 2008</a></li>
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<!--
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<a </a> main menu on plastic degradation
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<a href="https://2013.igem.org/Team:Washington/LightSensing">Light Sensing Experiments</a>
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<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Background">Background</a></li>
 +
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Cloning">Cloning</a></li>
 +
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Systems">Our Systems</a></li>
 +
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Methods">Methods</a></li>
 +
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Results">Results</a></li>
 +
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Sources">Scientific Sources</a></li>
 +
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Future">Future Plans</a></li>
 +
<li><a href="https://2013.igem.org/Team:Washington/LightSensing#Parts">Parts Submitted</a></li>
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<a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE">e.colightTune</a>
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<li><a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE#Background">Background</a></li>
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<li><a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE#Usage">App</a></li>
 +
<li><a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE#Benefits">Methods</a></li>
 +
<li><a href="https://2013.igem.org/Team:Washington/ECOLIGHTTUNE#Results">Results Summary</a></li>
 +
</ul>
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</li>
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<li>
 +
<a href="https://2013.igem.org/Team:Washington/Outreach">Outreach</a>
 +
<ul>
 +
<li><a href="https://2013.igem.org/Team:Washington/Outreach#Activities">Activities</a></li>
 +
<li><a href="https://2013.igem.org/Team:Washington/Outreach#Pictures">Pictures</a></li>
 +
</ul>
 +
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<li>
 +
<a href="https://2013.igem.org/Team:Washington/Protocols">Protocols</a>
 +
<ul>
 +
<li><a href="https://2013.igem.org/Team:Washington/Protocols#General_Protocols">General</a></li>
 +
<li><a href="https://2013.igem.org/Team:Washington/Protocols#Cloning_Protocols">Cloning</a></li>
 +
<li><a href="https://2013.igem.org/Team:Washington/Protocols#Assay">Light assays</a></li>
 +
</ul>
 +
</li>
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 +
<li>
 +
<a href="https://2013.igem.org/Team:Washington/MoreLinks">Links</a>
 +
<ul>
 +
<li><a href="https://2013.igem.org/Team:Washington/MoreLinks#Safety">Safety Form</a></li>
 +
<li><a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=Washington">Parts Registry</a></li>
 +
<li><a href="https://2013.igem.org/Team:Washington/MoreLinks#Documentation">Parts Documentation</a></li>
 +
 +
</ul>
 +
</li>
 +
 +
<li style="border-right:0; box-shadow:0px 0 0;">
 +
<a href="https://2013.igem.org/Team:Washington/Funding">Funding</a>
 +
</li>
 +
 +
</li>
 +
                                                                                               
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Revision as of 07:04, 7 September 2013


""

DNA Sequencing

  1. Add 10uL of miniprep DNA (at least 50ng/ul) + 5 uL of primer to a PCR Strip Tube. The only labels on the Tube should be AC01 ... ACXX (depending on the number of samples, XX)

Create a COPY of the genewiz_premix document (in folder) and rename it with today’s date.

  1. Enter your DNA Name (make sure it is not redundant and in order of wells you are submitting)
  2. DNA Type --> "Plasmid" (click the Type button to autofill the rest, same for all others)
  3. DNA Length --> "6000kb"
  4. Primer Name → i.e. "VF2" or "VR" (if you want forward or reverse, respectively)

Send Google doc to Advisor in sharing options.

  1. Print out receipt (Send to an advisor and we can print it out) and place labeled samples (AC01 ... ACXX) and order sheet in a Plastic Bag and then Drop Box
  2. When sequencing is completed you can view the results by logging in going to the appropriate link under "Recent Orders" on the lower right side of the screen

Retrieved from "http://2013.igem.org/Team:Washington/DNA_SEQUENCING"