Team:Peking/Lab

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<h1 id="ProjectName">AROMATICS BUSTED</h1>
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<h1 id="ProjectName">SAFETY</h1>
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                 <h1 id="ProjectSubname">A FAST, EASY AND ACCURATE METHOD TO DETECT TOXIC AROMATIC COMPOUNDS</h1>
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                 <h1 id="ProjectSubname">ARE WE BIOSAFE ENOUGH?</h1>
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             <p id="AbstractContent">As aromatic pollution levels rise, and along with it the environmental and health hazards that it presents, finding a better way of detecting these aromatic compounds becomes more and more important. Some prokaryotes, including <I>Escherichia coli</I> and <I>Pseudomonas putida</I>, naturally produce proteins capable of both detecting toxic aromatic molecules and regulating the transcription of corresponding catabolic genes. However, these naturally existing biosensors are limited by their detection range, expression leakage, and induction ratio.<br/><br/>Using these proteins, our team designed a series of improved aromatic sensors and combined them with related enzymes from their original catabolic pathways to increase the molecule detection range of these sensors. In order to semi-quantitatively measure the concentrations of target compounds, we constructed a biological band-pass circuit, enabling our sensors to detect the concentrations of target compounds within a certain range. In sum, we have been working on a fast, easy and accurate way to detect toxic aromatic compounds.</p>
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         <h1 id="Question1Title" class="QuestionTitle">Experimental Safety</h1>
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         <h2 id="Question1Subtitle" class="QuestionSubTitle">Would any of your project ideas raise safety issues in terms of:<br />researcher safety, public safety, or environmental safety?</h2>
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         <p id="Question1Answer" class="QuestionAnswer">The only organisms we worked with are Escherichia coli K 12 and Pseudomonas Putida MT 2, both of which are considered lab-safe. The main dangerous elements in this project are the target aromatic chemicals. The risks these aromatic compounds pose are precisely the reason that we are working with them. We took careful steps to protect ourselves while working with them in the lab.<br/> <br/><br/><br/><br/><br/><br/><br/> <br/><br/><br/><br/><br/><br/><br/> <br/><br/>                                                                                                                    <br/>Members who were working directly with these compounds wore particulate respirators for protection against inhalation. In addition to masks, lab members also wore lab coats and gloves to protect against absorption into the skin. After use, these compounds were disposed of by sending them off to the College of Chemistry for appropriate handling. 
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<br/>Containment is not a problem. If our E. coli or parts leaked into the environment, we do not foresee bacteria that fluoresce upon exposure to toxic aromatic compounds being an environmental or health hazard. Our parts would be very difficult to maliciously misuse, unless the intent is to maliciously detect compounds.</p>
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         <h1 id="Question2Title" class="QuestionTitle">BioBrick Parts Safety</h1>
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         <h2 id="Question2Subtitle" class="QuestionSubTitle">Do any of the new BioBrick parts (or devices) that you made this year raise safety issues? </h2>
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         <p id="Question2Answer" class="QuestionAnswer">Our BioBrick parts do not pose any safety issues.</p>
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         <h1 id="Question3Title" class="QuestionTitle">Safety Supervison</h1>
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         <h2 id="Question3Subtitle" class="QuestionSubTitle">Is there a local biosafety group, committee, or review board at your institution?</h2>
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         <p id="Question3Answer" class="QuestionAnswer">The 2013 Peking iGEM team complies with all the rules set forth by The Office of Laboratory and Equipment of Peking University strictly. We did not have direct contact with the office, however our project was closely monitored by team advisors and instructors, who ensured that laboratory risks were kept at a minimum.</p>
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         <h1 id="Question4Title" class="QuestionTitle">Suggestions</h1>
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        <h2 id="Question4Subtitle" class="QuestionSubTitle"> Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions?</h2>
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        <p id="Question4Answer" class="QuestionAnswer">A mandatory biosafety education video or brief class for all iGEM teammates, including a mechanism to ensure that all teammates involved in wet lab work complete the class, for example a verification code supplied upon completion that needs to be submitted by all team members in order for an iGEM team to be able to register for the jamboree. Having better-informed scientists is the best and fastest way to a safer competition.</p>
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<img id="AcknowledgementBox" src="https://static.igem.org/mediawiki/igem.org/c/c4/Peking_Sponsors.jpg">
<img id="AcknowledgementBox" src="https://static.igem.org/mediawiki/igem.org/c/c4/Peking_Sponsors.jpg">

Latest revision as of 07:54, 8 September 2013

SAFETY

ARE WE BIOSAFE ENOUGH?

Experimental Safety

Would any of your project ideas raise safety issues in terms of:
researcher safety, public safety, or environmental safety?

The only organisms we worked with are Escherichia coli K 12 and Pseudomonas Putida MT 2, both of which are considered lab-safe. The main dangerous elements in this project are the target aromatic chemicals. The risks these aromatic compounds pose are precisely the reason that we are working with them. We took careful steps to protect ourselves while working with them in the lab.

















Members who were working directly with these compounds wore particulate respirators for protection against inhalation. In addition to masks, lab members also wore lab coats and gloves to protect against absorption into the skin. After use, these compounds were disposed of by sending them off to the College of Chemistry for appropriate handling.
Containment is not a problem. If our E. coli or parts leaked into the environment, we do not foresee bacteria that fluoresce upon exposure to toxic aromatic compounds being an environmental or health hazard. Our parts would be very difficult to maliciously misuse, unless the intent is to maliciously detect compounds.

BioBrick Parts Safety

Do any of the new BioBrick parts (or devices) that you made this year raise safety issues?

Our BioBrick parts do not pose any safety issues.

Safety Supervison

Is there a local biosafety group, committee, or review board at your institution?

The 2013 Peking iGEM team complies with all the rules set forth by The Office of Laboratory and Equipment of Peking University strictly. We did not have direct contact with the office, however our project was closely monitored by team advisors and instructors, who ensured that laboratory risks were kept at a minimum.

Suggestions

Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions?

A mandatory biosafety education video or brief class for all iGEM teammates, including a mechanism to ensure that all teammates involved in wet lab work complete the class, for example a verification code supplied upon completion that needs to be submitted by all team members in order for an iGEM team to be able to register for the jamboree. Having better-informed scientists is the best and fastest way to a safer competition.

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