Team:BYU Provo/Notebook/LargePhage/Springexp/Period2/Explist

From 2013.igem.org

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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage May - June Notebook: April 29 - May 12 Experiment Listing'''</font>
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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Large Phage May - June Notebook: May 13 - May 26 Experiment Listing'''</font>
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<font size="4"> '''5.3 T7 Phage Selection Method Test''' </font>
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<font size="4"> '''Phage Liquid Culture Mutagenesis''' </font>
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In this experiment, we aimed to test whether or not we can select for smaller phages using concentrated top agar.
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Description of the experiment
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Detailed procedure: [[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 T7 phage selection method test|5.3 T7 Phage Selection Method Test]]
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Detailed procedure: [[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period1/Exp/Team:BYU_Provo/Phage Liquid Culture Mutagenesis]]
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<font size="4"> '''5.3 T7 Phage Amplification/Purification''' </font>
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<font size="4"> '''UV Selection Test''' </font>
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In this experiment, we aimed to establish a basic protocol for amplifying and purifying phage.
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Description of the experiment  
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Detailed procedure: [[Team:BYU Provo/Notebook/LargePhage/Springexp/Period1/Exp/Team:BYU Provo/5.3 Phage Amplification/Purification|5.3 T7 Phage Amplification/Purification]]
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Detailed procedure: [[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period1/Exp/Team:BYU_Provo/UV Selection Test]]
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<font size="4"> '''5.5 Amplification From a Plaque Test''' </font>
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In this experiment, we aimed to test whether or not we can amplify a phage plaque using liquid culture
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Detailed procedure: [[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period1/Exp/Team:BYU_Provo/5.5_Amplification_from_a_plaque_test|5.5 Amplification From a Plaque Test]]
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<font size="4"> '''5.6 T7+ Liquid Culture Phage Concentration Test ''' </font>
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In this experiment, we aimed to test whether or not starting phage liquid culture with different concentrations of phage will affect the overall yield of phage progeny.
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Detailed procedure: [[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period1/Exp/Team:BYU_Provo/5.6_T7%2B_Liquid_Culture_Phage_Concentration_Test|5.6 T7+ Liquid Culture Phage Concentration Test ]]
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<font size="4"> '''5.9 T7 Selection Method Test #2''' </font>
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We performed this experiment to familiarize ourselves with x8 agar.
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Detailed procedure: [[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period1/Exp/5.9_T7_selection_method_test|5.9 T7 Selection Method Test #2]]
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<font size="4"> '''5.9 T7+ Liquid Culture Phage Concentration Test #2''' </font>
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We repeated [[Team:BYU_Provo/Notebook/LargePhage/Springexp/Period1/Exp/Team:BYU_Provo/5.6_T7%2B_Liquid_Culture_Phage_Concentration_Test|5.6 T7+ Liquid Culture Phage Concentration Test ]] to test for whether 1uL will give us even higher phage titer
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Detailed procedure: [[Team:BYU Provo/Notebook/LargePhage/Springexp/Period1/Exp/5.9 T7+ Liquid Culture Phage Concentration Test|5.9 T7+ Liquid Culture Phage Concentration Test #2]]
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Latest revision as of 21:53, 5 June 2013


Large Phage May - June Notebook: May 13 - May 26 Experiment Listing



Overview
March-April
May-June
July-August
September-October

Phage Liquid Culture Mutagenesis

Description of the experiment

Detailed procedure: Team:BYU_Provo/Notebook/LargePhage/Springexp/Period1/Exp/Team:BYU_Provo/Phage Liquid Culture Mutagenesis


UV Selection Test

Description of the experiment

Detailed procedure: Team:BYU_Provo/Notebook/LargePhage/Springexp/Period1/Exp/Team:BYU_Provo/UV Selection Test


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