Team:UNITN-Trento/Notebook/Labposts/06/45

From 2013.igem.org

(Difference between revisions)
(Created page with "{ "date" : "2013-06-20", "author" : "emil-gabriele", "title" : "Linearization of psB1C3(Failed) and screening of SAM sinthethase", "content" : "<html>We quantified yesterday ...")

Revision as of 07:44, 9 September 2013

{ "date" : "2013-06-20", "author" : "emil-gabriele", "title" : "Linearization of psB1C3(Failed) and screening of SAM sinthethase", "content" : "We quantified yesterday inocula (1:1, 1:2, 1:4 of SAMsynthetase and R0010, both in pSB1C3).

Quantification results
SampleQuantification (ng/µl)
1:1 A293.0
1:1 B135.7
1:1 C178.0
1:1 D139.3
1:1 E262.0
1:1 F147.5
1:1 G212.5
1:1 H210.2
1:2 A141.0
1:2 B123.6
1:4 A207.8
1:4 B127.2
R0010 A131.7
R0010 B119.6
R0010 C371.0
R0010 D307.9
R0010 E313.8
Then, we screened the products of ligation (1:1, 1:2, 1:4 and R0010): digestion with EcoRI-HF and PstI-HF following the digestion protocol and agarose gel 1%.
1% Agarose Gel Image
Loading Scheme
Empty
1kb ladder
Empty
1:1 D
1:1 E
Empty
1:2 B
Empty
1:4 A
Empty
R0010 E
1% Agarose Gel Image
Loading scheme
1kb ladder
R0010 A
R0010 B
R0010 C
R0010 D
R0010 E
1% Agarose Gel Image
Loading scheme
R0010 B
R0010 C
R0010 D
1kb ladder
1:4 A
1:4 B
1:2 A
1:2 B
1.5% Agarose Gel Image
Loading scheme
1kb ladder
R0010 A
R0010 B
R0010 C
R0010 D
R0010 E
In these images we can only see the band of the backbone and no insert!", "tags" : "SAMsynthetase" }

Retrieved from "http://2013.igem.org/Team:UNITN-Trento/Notebook/Labposts/06/45"