09/09/13

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==Preparation of overnight broth==
==Preparation of overnight broth==
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* Following on from [[07/09/13]] result,overnight broth was prepared yesterday from the single colonies that had grown on each of the 2 plates shown on [[07/09/13]].
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* Following on from [[06/09/13]] result,overnight broth was prepared yesterday from the single colonies that had grown on each of the 2 plates shown on [[06/09/13]].

Revision as of 09:56, 9 September 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

Preparation of overnight broth

  • Following on from 06/09/13 result,overnight broth was prepared yesterday from the single colonies that had grown on each of the 2 plates shown on 06/09/13.


Miniprep of broth

  • The broth, which contains cells that have been transformed with pSB1C3/TOD genes, was minipreped using the Omega Bio-Tek kit.
  • This would enable us to confirm which cells have the recircularised plasmid and those with the ligated product.

Measurement of DNA concentration