Team:Groningen/protocols/GelElectrophoresis

From 2013.igem.org

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<div class="mainContent">
<div class="mainContent">
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<h1>Gel electrophoresis</h1>
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<h2>Gel electrophoresis</h2>
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<h3>Materials:</h3>
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<h5>Materials:</h5>
<ul type="square">
<ul type="square">
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<li></li>
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<li>TBE buffer 1x</li>
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<li></li>
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<li>0.8 or 1.5% agarose gel</li>
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<li></li>
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<li>2x Loading Dye
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<li></li>
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<li>DNA samples</li>
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<li></li>
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<li>DNA 1 kb GeneRuler of Fermentas</li>
</ul>
</ul>
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<h3>:</h3>
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<p>
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<h5>Procedure:</h5>
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<br>All the reagents are added following the order listed in the table above.
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- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
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<br>After the reaction is ready mix the content of the tube and spin it down.
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<br>- Load the samples on a 0.8% or 1.5% agarose gel
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<br>The tubes are incubated for 1h at 37&deg;C.
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<br>- Load the 1kb GeneRuler of Fermentas on gel
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<br>
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<br>- Run the gel at 90V for 24-45 minutes to separate the bands.
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Latest revision as of 12:05, 9 September 2013

Gel electrophoresis

Materials:
  • TBE buffer 1x
  • 0.8 or 1.5% agarose gel
  • 2x Loading Dye
  • DNA samples
  • DNA 1 kb GeneRuler of Fermentas

Procedure:
- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
- Load the samples on a 0.8% or 1.5% agarose gel
- Load the 1kb GeneRuler of Fermentas on gel
- Run the gel at 90V for 24-45 minutes to separate the bands.