Team:Groningen/protocols/GelElectrophoresis

From 2013.igem.org

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<div class="mainContent">
<div class="mainContent">
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<h1>Gel electrophoresis</h1>
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<h2>Gel electrophoresis</h2>
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<h3>Materials:</h3>
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<h5>Materials:</h5>
<ul type="square">
<ul type="square">
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<li>Power supply</li>
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<li>TBE buffer 1x</li>
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<li>Agarose gel</li>
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<li>0.8 or 1.5% agarose gel</li>
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<li>Tray</li>
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<li>2x Loading Dye
<li>DNA samples</li>
<li>DNA samples</li>
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<li>DNA Ladder</li>
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<li>DNA 1 kb GeneRuler of Fermentas</li>
</ul>
</ul>
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<h3>Procedure:</h3>
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<p>
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All the DNA samples are mixed 1:1 ratio with Loading Dye 6x.
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<h5>Procedure:</h5>
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<br>The samples are loaded on agarose gel 0.8% or 1.5% (diluted in TBE buffer 1x).
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- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
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<br>Beside the samples the DNA ladder is loaded.
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<br>- Load the samples on a 0.8% or 1.5% agarose gel
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<br>90V electric potential is applied to the gel as long as the bands are apart from each other (30-45 minutes).
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<br>- Load the 1kb GeneRuler of Fermentas on gel
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<br>The gel are run in TBE buffer 1x.
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<br>- Run the gel at 90V for 24-45 minutes to separate the bands.
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Latest revision as of 12:05, 9 September 2013

Gel electrophoresis

Materials:
  • TBE buffer 1x
  • 0.8 or 1.5% agarose gel
  • 2x Loading Dye
  • DNA samples
  • DNA 1 kb GeneRuler of Fermentas

Procedure:
- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
- Load the samples on a 0.8% or 1.5% agarose gel
- Load the 1kb GeneRuler of Fermentas on gel
- Run the gel at 90V for 24-45 minutes to separate the bands.