Team:Groningen/protocols/GelElectrophoresis

From 2013.igem.org

(Difference between revisions)
 
(15 intermediate revisions not shown)
Line 22: Line 22:
<div class="mainContent">
<div class="mainContent">
-
<h1>Gel electrophoresis</h1>
+
<h2>Gel electrophoresis</h2>
-
<h3>Materials:</h3>
+
<h5>Materials:</h5>
<ul type="square">
<ul type="square">
-
<li>Power supply</li>
+
<li>TBE buffer 1x</li>
-
<li>Agarose gel</li>
+
<li>0.8 or 1.5% agarose gel</li>
-
<li>Tray</li>
+
<li>2x Loading Dye
<li>DNA samples</li>
<li>DNA samples</li>
-
<li>DNA Ladder</li>
+
<li>DNA 1 kb GeneRuler of Fermentas</li>
</ul>
</ul>
-
<h3>Procedure:</h3>
+
<p>
-
All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
+
<h5>Procedure:</h5>
-
<br>The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb gene ruler of Fermentas.
+
- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
-
<br>A 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.
+
<br>- Load the samples on a 0.8% or 1.5% agarose gel
-
<br>The gel is run in TBE buffer 1x.
+
<br>- Load the 1kb GeneRuler of Fermentas on gel
 +
<br>- Run the gel at 90V for 24-45 minutes to separate the bands.
</div>
</div>

Latest revision as of 12:05, 9 September 2013

Gel electrophoresis

Materials:
  • TBE buffer 1x
  • 0.8 or 1.5% agarose gel
  • 2x Loading Dye
  • DNA samples
  • DNA 1 kb GeneRuler of Fermentas

Procedure:
- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
- Load the samples on a 0.8% or 1.5% agarose gel
- Load the 1kb GeneRuler of Fermentas on gel
- Run the gel at 90V for 24-45 minutes to separate the bands.