Team:Groningen/protocols/GelElectrophoresis

From 2013.igem.org

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<div class="mainContent">
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<h1>Gel electrophoresis</h1>
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<h2>Gel electrophoresis</h2>
<h5>Materials:</h5>
<h5>Materials:</h5>
<ul type="square">
<ul type="square">
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<li>Power supply</li>
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<li>TBE buffer 1x</li>
<li>0.8 or 1.5% agarose gel</li>
<li>0.8 or 1.5% agarose gel</li>
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<li>Gel tray</li>
 
<li>2x Loading Dye
<li>2x Loading Dye
<li>DNA samples</li>
<li>DNA samples</li>
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<li>DNA 1kb GeneRuler of Fermentas</li>
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<li>DNA 1 kb GeneRuler of Fermentas</li>
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</ul>
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<p>
<h5>Procedure:</h5>
<h5>Procedure:</h5>
- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
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<br>- Load the samples on gel
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<br>- Load the samples on a 0.8% or 1.5% agarose gel
<br>- Load the 1kb GeneRuler of Fermentas on gel
<br>- Load the 1kb GeneRuler of Fermentas on gel
<br>- Run the gel at 90V for 24-45 minutes to separate the bands.
<br>- Run the gel at 90V for 24-45 minutes to separate the bands.
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<p>All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
 
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<br>The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb GeneRuler of Fermentas.
 
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<br>The gel is placed in TBE buffer 1x and a 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.
 
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Latest revision as of 12:05, 9 September 2013

Gel electrophoresis

Materials:
  • TBE buffer 1x
  • 0.8 or 1.5% agarose gel
  • 2x Loading Dye
  • DNA samples
  • DNA 1 kb GeneRuler of Fermentas

Procedure:
- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
- Load the samples on a 0.8% or 1.5% agarose gel
- Load the 1kb GeneRuler of Fermentas on gel
- Run the gel at 90V for 24-45 minutes to separate the bands.