Team:BYU Provo/Notebook/SmallPhage/Springexp/Period4/Dailylog

From 2013.igem.org

(Difference between revisions)
 
(2 intermediate revisions not shown)
Line 14: Line 14:
<font color="#333399" size="3" font face="Calibri">
<font color="#333399" size="3" font face="Calibri">
-
: [[Team:BYU_Provo/Small_Phage|Overview]]
+
<font size = "4">
 +
 
 +
: <u> '''Small Phage''' </u> </font>
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
Line 71: Line 73:
<font size="4"> '''6/27/13''' </font>
<font size="4"> '''6/27/13''' </font>
-
- Started the mutagenesis procedure for [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.27_Mutagen_Concentration_Test_-_Third_Protocol|6.27_Mutagen_Concentration_Test_-_Third_Protocol]]
+
- Started the mutagenesis procedure for [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.27_Mutagen_Concentration_Test_-_Third_Protocol|6.27 Mutagen Concentration Test - Third Protocol]]
<br>
<br>
-
<font size="4"> '''6/5/13''' </font>
+
<font size="4"> '''6/28/13''' </font>
-
- Discussed plans for the selection process of [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 Mutagen Concentration Experiment|5.20 Mutagen Concentration Experiment]] and calculated the needed volume of agar, overnight, and phage
+
- Performed first round of titer for [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.27_Mutagen_Concentration_Test_-_Third_Protocol|6.27 Mutagen Concentration Test - Third Protocol]]. This includes 0, -2, -4, -6, and -8 for 0ug, 100ug, 200ug, and 500ug mutagen concentration.
-
 
+
-
- Made 500mL x8 agar
+
-
 
+
-
- Performed dilution series to generate enough 200ug -4 phage stock for selection.
+
<br>
<br>
-
 
-
<font size="4"> '''6/6/13''' </font>
 
-
 
-
- Started approximately 50mL of BL21 liquid culture overnight.
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''6/7/13''' </font>
 
-
 
-
- Performed the selection process of  [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 Mutagen Concentration Experiment|5.20 Mutagen Concentration Experiment]]. Specifically, we plated 45 plates of 200ug mutagen, -4 phage dilution using x8 agar. 5 plates of 0ug, -3 phage dilution were also done a control.
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''6/9/13''' </font>
 
-
 
-
- Started 21mL of BL21 overnight.
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''6/10/13''' </font>
 
-
 
-
- Made 1250mL of x8 top agar
 
-
 
-
- Attempted to amplify phage from larger plaques in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 Mutagen Concentration Experiment|5.20 Mutagen Concentration Experiment]]
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''6/11/13''' </font>
 
-
 
-
- Started approximately 70mL of BL21 liquid culture overnight
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''6/12/13''' </font>
 
-
 
-
- Perfected protocol for applying mutagen to phage
 
-
 
-
- Started [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Perfected Protocol]]
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''6/13/13''' </font>
 
-
 
-
- Started approximately 30mL of BL21 liquid culture overnight.
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''6/14/13''' </font>
 
-
 
-
- Titered the mutagenesis product from Wednesday as part of [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Perfected Protocol]]
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''6/16/13''' </font>
 
-
 
-
- Started approximately 100mL of BL21 liquid culture overnight.
 
-
 
-
<br>
 
-
 
</font>
</font>

Latest revision as of 15:31, 9 September 2013


Small Phage May - June Notebook: June 17 - June 30 Daily Log



Small Phage
March-April
May-June
July-August
September-October


6/17/13

- Plated 5 controls and 50 selection plates (500ug at -2) as part of selection 1 in 6.12 Mutagen Concentration Test - Second Protocol


6/18/13

- Started approximately 50mL of BL21 liquid culture overnight.


6/19/13

- We performed Large Plaque Confirmation 1 as part of 6.12 Mutagen Concentration Test - Second Protocol


6/23/13

- Started 40mL of BL21 liquid culture overnight.


6/24/13

- Made more x8 top agar. - Because the large plaque confirmation 1 had contamination from overnight (the LB was contaminated the next day), we redid the confirmation procedure: Large Plaque Confirmation 2 in 6.12 Mutagen Concentration Test - Second Protocol.


6/26/13

- Made about 50ml of BL21 liquid culture overnight


6/27/13

- Started the mutagenesis procedure for 6.27 Mutagen Concentration Test - Third Protocol


6/28/13

- Performed first round of titer for 6.27 Mutagen Concentration Test - Third Protocol. This includes 0, -2, -4, -6, and -8 for 0ug, 100ug, 200ug, and 500ug mutagen concentration.


Retrieved from "http://2013.igem.org/Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period4/Dailylog"