Team:Groningen/Labwork/11 September 2013

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<Br>There were no mutations.  
<Br>There were no mutations.  
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<br>BBa_K1085014+P-RFP, only one primer bound in the wrong direction, implying that the promoter is inserted in the wrong direction.
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<br>BBa_K1085014+P-RFP, only one primer bound in the wrong direction, implying that the promoter is inserted in the wrong direction. The other primer didn't bound at all.
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checked colonies stabbed on LB/starch/cm plates, with lugols solution. No halo's were visible implying that the Bacillus strain has an insert in the <i>amyE<i/> locus.
    
    

Revision as of 10:12, 11 September 2013

Sebas

Checked sequenced plasmids. BBa_K1085014+GFPdsm and BBa_K1085014+GFP0840 had both the promoter orientated in the right direction.
There were no mutations.

BBa_K1085014+P-RFP, only one primer bound in the wrong direction, implying that the promoter is inserted in the wrong direction. The other primer didn't bound at all.

checked colonies stabbed on LB/starch/cm plates, with lugols solution. No halo's were visible implying that the Bacillus strain has an insert in the amyE locus.