Team:Groningen/Labwork/12 September 2013

From 2013.igem.org

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<br>Saw colonies on the plates of complete transformation of the &Delta;CheY mutant to our <i>B. subtilis</i> 168 strain.
<br>Saw colonies on the plates of complete transformation of the &Delta;CheY mutant to our <i>B. subtilis</i> 168 strain.
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<h2>Inne</h2>
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Prepared the samples of biobrick BBa_k1085012 and BBa_k1085010 for sequencing. 4 samples were prepared.
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<table border ="1">
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<tr>
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<td>Biobrick</td>
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<td>Primer</td>
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</tr>
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<tr>
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<td>BBa_k1085012 5 uL</td>
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<td>VF2 5 uL</td>
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</tr>
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<tr>
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<td>BBa_k1085012 5 uL</td>
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<td>VR 5 uL</td>
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</tr>
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<tr>
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<td>BBa_1085010</td>
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<td>VF2 5 u/L</td>
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</tr>
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<tr>
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<td>BBa_1085010</td>
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<td>VR 5 u/L</td>
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</tr>
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</table>
</div>
</div>

Revision as of 12:48, 12 September 2013

Sebas

Claudio

The plates pSB1C3-S1-S5-S5 and pSB1C3-S2-S5-S5 showed thousands of colonies.
Colony PCR was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 58°C).
The samples were checked on agarose gel 0.8%.

Mirjam

Did a ligation of the silk plus signal sequences (made by Sander) to the registration backbone.
Saw colonies on the plates of complete transformation of the ΔCheY mutant to our B. subtilis 168 strain.

Inne

Prepared the samples of biobrick BBa_k1085012 and BBa_k1085010 for sequencing. 4 samples were prepared.
Biobrick Primer
BBa_k1085012 5 uL VF2 5 uL
BBa_k1085012 5 uL VR 5 uL
BBa_1085010 VF2 5 u/L
BBa_1085010 VR 5 u/L