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Team:Groningen/Project/secretion
From 2013.igem.org
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Revision as of 11:19, 13 September 2013
Silk production
We developed 12 different types of spider silks (figure 1). We have one spider silk protein with a N and C terminus, a spider silk without a N and C terminus and a spidersilk without one block (explain the difference). A codon optimization script was developed to codon optimize the spider silk for b. subtilis. Codon optimization (state why we did codon optimization and link towards the codon optimazation page on the wiki for all the details). Every silk gene was codon optimized twice with different results. All the codon optimized silk genes were ordered and made synthetically (figure 2).
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| Figure 1, our spider silks |
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| Figure 2: Not sure if this image needs to be on our site, if so please explain thoroughly. |
The silk genes are provided with an signal peptide and a strep tag. The signal peptide will ensure the secretion of silk by the natural pathways of b. subtilis (figure 3). The strep tag will be used for our coating mechanism.
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| Figure 3: the secretion pathway of silk |
