Team:Tianjin

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<li style="margin-left:200px;"><a href="https://2013.igem.org/Team:Tianjin" onmouseover="mopen('m1')" onmouseout="mclosetime()">Home</a>
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<li><a href="https://2013.igem.org/Team:Tianjin/Project" onmouseover="mopen('m2')" onmouseout="mclosetime()">Project</a>
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<li><a href=" https://2013.igem.org/Team:Tianjin/Safety " onmouseover="mopen('m3')" onmouseout="mclosetime()">Safty</a>
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<li><a href=" https://2013.igem.org/Team:Tianjin/Protocol " onmouseover="mopen('m5')" onmouseout="mclosetime()">Protocol</a>
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    <li><a href=" https://2013.igem.org/Team:Tianjin/Attributions " onmouseover="mopen('m10')" onmouseout="mclosetime()">Attributions</a>
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                <li><a href="#">4</a></li>
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                <li><a href="#">5</a></li>
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<p style="font-size:24px;font-family:Arial, Helvetica, sans-serif;font-weight:bold">Abstract</p>
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<hr />
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<p style="font-size:14px;font-family:Arial, Helvetica, sans-serif;line-height:150%; word-spacing:0.3em;"><b>B</b>iosynthesized alkanes are promising candidates for drop-in replacement of petroleum. Our goal is to optimize the pathway of alkane synthesis and get high productivity. We picked out several key points in the pathway from glucose to various alkanes and generated a large number of strains with different productivity. In order to select out the ones with highest productivity, we designed a device named AlkFinder. The core of AlkFinder is a biosensor which can recognize sensitively alkane molecules within the cell and turn on the expression of downstream genes functioning as selection module. With AlkFinder, we managed to select the optimized genes of the pathway effectively and efficiently. Besides, we also increased the efflux of biofuel molecule to enhance Escherichia coli's resistance against alkanes. 
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<div id="Enlarge">
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<a href="#"  title="Enlarge"><img src="images/loading.gif" width="15" height="11" alt="" /></a></div>
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<b>Figure 1.</b> Word "RNA" (from "<a href="#" rel="nofollow">http://www.mfpl.ac.at</a>")</div></div></div>
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{|align="justify"
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<div style="height:300px;">
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|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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<p style="font-size:24px;font-family:Arial, Helvetica, sans-serif;font-weight:bold">Item2</p>
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|[[Image:Tianjin_logo.png|200px|right|frame]]
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<div style="width:300px;float:left;margin:20px 10px 10px 10px; ">
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<a href="#" ><img alt="" src="images/001.jpg" width="300" height="192"/></a> <br/>
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''Tell us more about your project. Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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<p><center>The discription of picture 1.<br/><a href="#">More about...</a></center></p>
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|[[Image:Tianjin_team.png|right|frame|Your team picture]]
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|-
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<div style="width:300px;float:left;margin:20px 10px 10px 10px; ">
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<a href="#" ><img alt="" src="images/002.jpg" width="300" height="192"/></a> <br/>
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|align="center"|[[Team:Tianjin | Team Tianjin]]
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<p><center>The discription of picture 2.<br/><a href="#">More about..</a></center></p>
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|}
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</div>
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<div style="width:300px;float:left;margin:20px 10px 10px 10px; ">
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<a href="#" ><img alt="" src="images/003.jpg" width="300" height="192"/></a> <br/>
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<p><center>The discription of picture 3.<br/><a href="#">More about...</a></center></p>
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</div>
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<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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<div style="height:500px;width:630px;float:left;">
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!align="center"|[[Team:Tianjin|Home]]
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<p style="font-size:24px;font-family:Arial, Helvetica, sans-serif;font-weight:bold">Item3</p>
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!align="center"|[[Team:Tianjin/Team|Team]]
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<hr />
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Tianjin Official Team Profile]
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<div style="width:190px;float:left;margin:20px 10px 10px 10px;">
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!align="center"|[[Team:Tianjin/Project|Project]]
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<a href="#" ><img alt="1" src="images/001.jpg" width="190px" height="150px"/></a> <br/>
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!align="center"|[[Team:Tianjin/Parts|Parts Submitted to the Registry]]
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<p><center>The discription of picture 1.<br/><a href="#">More about...</a></center></p>
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!align="center"|[[Team:Tianjin/Modeling|Modeling]]
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</div>
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!align="center"|[[Team:Tianjin/Notebook|Notebook]]
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<div style="width:190px;float:left;margin:20px 10px 10px 10px; ">
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!align="center"|[[Team:Tianjin/Safety|Safety]]
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<a href="#" ><img alt="" src="images/002.jpg" width="190px" height="150px"/></a> <br/>
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!align="center"|[[Team:Tianjin/Attributions|Attributions]]
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<p><center>The discription of picture 2.<br/><a href="#">More about..</a></center></p>
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|}
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</div>
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<div style="width:190px;float:left;margin:20px 10px 10px 10px; ">
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<a href="#" ><img alt="" src="images/003.jpg" width="190px" height="150px"/></a> <br/>
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<p><center>The discription of picture 3.<br/><a href="#">More about...</a></center></p>
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</div>
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</div>
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<div style="float:left;width:280px;height:500px; margin-left:50px;">
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<p style="font-size:24px;font-family:Arial, Helvetica, sans-serif;font-weight:bold">Item4</p>
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<hr />
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<div style="margin-top:20px;padding-left:10px;padding-right:10px;">
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<p style="font-size:14px;font-family:Arial, Helvetica, sans-serif;line-height:150%; word-spacing:0.3em;">Biosynthesized alkanes are promising candidates for drop-in replacement of petroleum. Our goal is to optimize the pathway of alkane synthesis and get high productivity. We picked out several key points in the pathway from glucose to various alkanes and generated a large number of strains with different productivity. In order to select out the ones with highest productivity, we designed a device named AlkFinder. The core of AlkFinder is a biosensor which can recognize sensitively alkane molecules within the cell and turn on the expression of downstream genes functioning as selection module.</p>
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<div class="icon-slide" style="float:left;">
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<img src='images/icon-Home.jpg' alt='' width="192px" height="192px"/>
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<a href="https://2013.igem.org/Team:Tianjin"><div>
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    <b></b>
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    <br/><br/><br/><br/>
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    <span>
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<center>Home</center>
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    </span>
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</div></a>
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</div> <!-- end slide -->
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<div class="icon-slide" style="float:left;">
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<img src='images/icon-Project.jpg' alt='' width="192px" height="192px"/>
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<a href="https://2013.igem.org/Team:Tianjin/Project "><div>
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    <b></b>
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    <br/><br/><br/><br/>
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    <span>
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<center>Project</center>
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<img src='images/icon-Safety.jpg' alt='' width="192px" height="192px"/>
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<a href="https://2013.igem.org/Team:Tianjin/Safety "><div>
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    <b></b>
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    <br/><br/><br/><br/>
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    <span>
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<center>Safety</center>
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    </span>
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<a href="https://2013.igem.org/Team:Tianjin/Modeling "><div>
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    <b></b>
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    <br/><br/><br/><br/>
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<center>Modling</center>
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    </span>
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<div class="icon-slide">
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<img src='images/icon-Protocol.jpg' alt='' width="192px" height="192px"/>
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<a href="https://2013.igem.org/Team:Tianjin/Protocol "><div>
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    <b></b>
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    <br/><br/><br/><br/>
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    <span>
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<center>Protocol</center>
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</div></a>
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<p style="color:#FFF;padding-top:10px;"><center><h1>About us</h1></center></p>
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<br />
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E-mail：austinamens@gmail.com | Address:Building No.20, No.92 Weijin Road, Tianjin, China | Zip-code：300072
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<br />Copyright 2013 © Tianjin University iGEM Team
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Revision as of 01:33, 14 September 2013

Abstract

Biosynthesized alkanes are promising candidates for drop-in replacement of petroleum. Our goal is to optimize the pathway of alkane synthesis and get high productivity. We picked out several key points in the pathway from glucose to various alkanes and generated a large number of strains with different productivity. In order to select out the ones with highest productivity, we designed a device named AlkFinder. The core of AlkFinder is a biosensor which can recognize sensitively alkane molecules within the cell and turn on the expression of downstream genes functioning as selection module. With AlkFinder, we managed to select the optimized genes of the pathway effectively and efficiently. Besides, we also increased the efflux of biofuel molecule to enhance Escherichia coli's resistance against alkanes.

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Biosynthesized alkanes are promising candidates for drop-in replacement of petroleum. Our goal is to optimize the pathway of alkane synthesis and get high productivity. We picked out several key points in the pathway from glucose to various alkanes and generated a large number of strains with different productivity. In order to select out the ones with highest productivity, we designed a device named AlkFinder. The core of AlkFinder is a biosensor which can recognize sensitively alkane molecules within the cell and turn on the expression of downstream genes functioning as selection module.





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