Labsafety

The lab we work in is classified as BSL 1 (biosafety level 1), according to the [http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=CELEX:32000L0054:en:NOT|European Union Directive 2000/54/EG] and the German "Gesetz zur Regelung der Gentechnik ([http://www.gesetze-im-internet.de/gentg/| GenTG])" (law for the regulation of genetic engineering). There is a total of four Biosafety levels, with BSL 1 being the lowest and BSL 4 being the highest. This classification of the respective Biosafety levels is very similar to the one given in the [http://www.who.int/csr/resources/publications/biosafety/en/Biosafety7.pdf| World Health Organization (WHO) Laboratory Biosafety Manual]. Work inside a BSL 1 lab, such as ours, involves no devices that are potentially harmful to the researchers if they act according to the general precautionary measures. Especially, no pathogenic organisms are used.

A regular safety briefing and a lecture about the legal basics concerning biotechnology and genetic engineering are basic elements of our education at TU Munich. In this context, the handling of biological material, dangerous aspects of chemicals and the circumstances and protocols at the lab we work in are explained. Additionally a special safety briefing was held for all iGEM students by Dr. Martin Schlapschy who is the responsible person for lab safety

All of us have worked in laboratories before and have a certain experience withe biological parts and chemicals. When we are unsure about the safety measures that have tob e taken when handling certain chemicals and devices, we always have the support of our instrucutrs and the researchers working at Prof. Skerras lab.

Safety precautions during molecular biology experiments

Just like in every other biochemical laboratory, there are substances and devices in our lab which are potentially dangerous. These are three examples of how these situations are handled in our lab.

1. In every laboratory of molecular biology, specific chemicals are required for staining of DNA, in order to make it visible on agarose gels. Most of them directly intercalate into double-stranded DNA, making them carcinogenic. The substance we use is ethidium bromide. To prevent skin contact, we wear protective gloves made from nitrile rubber and change them frequently to prevent contamination with ethidium bromide. All gels and materials that came into contact with ethidium bromide are disposed of seperately. This is done in order to prevent their unintended leakage into the environment with subsequent harm to humans, animals and plants.

2. Methods of molecular biology often require strong acids or bases, like hydrochloric acid, or toxic substances such as methanol. We handle them with extreme caution under a fume hood and disposed of them separately.

3. Many devices in the lab can be potentially dangerous towards researchers if they are used carelessly or in the wrong way. One example for this are lamps emitting ultraviolet radiation, which can be cancerogenic and cause damage to the eyes. When dealing with UV radiation, wealways wear safety helmets made out of plexiglas. Being aware of the potential harm caused by a device allows the researcher to protect him-/herself appropriately.

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.