Team:Nanjing-China/Notebook

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
 
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
!align="center"|[[Team:Nanjing-China|Home]]
!align="center"|[[Team:Nanjing-China|Home]]
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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
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===1. Extraction of mRNA===
 +
:Kit
 +
 
 +
===2. RT-PCR===
 +
:Kit
 +
 
 +
===3.PCR===
 +
====(1) Colony PCR====
 +
:{|border=0
 +
|width="125"|2×PrimeStar
 +
|width="125"|12.5μl
 +
|-
 +
|Forward primer||1~2.5μl
 +
|-
 +
|Reverse primer||1~2.5μl
 +
|-
 +
|Bacteria solution||1~2μl
 +
|-
 +
|dd water||up to 25μl
 +
|}
 +
 
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:{|border=0
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|width="75"|Step 1
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|width="200"|94℃
 +
|-
 +
|Step 2||94℃
 +
|-
 +
|Step 3||Annealing temperature
 +
|-
 +
|Step 4||72℃
 +
|-
 +
|Step 5||goto step2, 25~35 cycles
 +
|-
 +
|Step 6||72℃
 +
|-
 +
|Pause at 4℃||
 +
|}
 +
 
 +
 
 +
====(2) PCR with DNA as template====
 +
:{|border=0
 +
|width="125"|2×PrimeStar
 +
|width="125"|12.5μl
 +
|-
 +
|Forward primer||1μl
 +
|-
 +
|Reverse primer||1μl
 +
|-
 +
|Template DNA||0.5μl
 +
|-
 +
|dd water||up to 25μl
 +
|}
 +
 
 +
:{|border=0
 +
|width="75"|Step 1
 +
|width="200"|94℃
 +
|-
 +
|Step 2||94℃
 +
|-
 +
|Step 3||Annealing temperature
 +
|-
 +
|Step 4||72℃
 +
|-
 +
|Step 5||goto step2, 25~35 cycles
 +
|-
 +
|Step 6||72℃
 +
|-
 +
|Pause at 4℃||
 +
|}
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:Gel running and purify the product.

Latest revision as of 15:43, 16 September 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions


Contents

1. Extraction of mRNA

Kit

2. RT-PCR

Kit

3.PCR

(1) Colony PCR

2×PrimeStar 12.5μl
Forward primer1~2.5μl
Reverse primer1~2.5μl
Bacteria solution1~2μl
dd waterup to 25μl
Step 1 94℃
Step 294℃
Step 3Annealing temperature
Step 472℃
Step 5goto step2, 25~35 cycles
Step 672℃
Pause at 4℃


(2) PCR with DNA as template

2×PrimeStar 12.5μl
Forward primer1μl
Reverse primer1μl
Template DNA0.5μl
dd waterup to 25μl
Step 1 94℃
Step 294℃
Step 3Annealing temperature
Step 472℃
Step 5goto step2, 25~35 cycles
Step 672℃
Pause at 4℃
Gel running and purify the product.
Retrieved from "http://2013.igem.org/Team:Nanjing-China/Notebook"