Latest revision as of 20:59, 16 September 2013

07 June 2013

Kristian

->20 g of LB in every 1 L of distilled water. Autoclaved medium

->in 1 of these L, 14 g of agar were added for making LB solid medium

Colonies of Top 10 E.coli were streaked on two plates
Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)

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-==lab 208==
+{{:Team:DTU-Denmark/Templates/StartPage|07 June 2013}}
-=== main purposes today ===
+=lab 208=
+<hr/>
+== Main purposes today ==
+<hr/>
 *helloworld-project
 *prepare solutions
 *[[Team:DTU-Denmark/Methods/Autoclaving|autoclaving]]
+*plate ''E.coli''
-===who were in the lab===
+==Who was in the lab==
+<hr/>
 Kristian
-===Procedure===
+==Procedure==
+<hr/>
 #1M CaCl_2 (200ml) weighed off 22.206 g CaCl_2
-#50% glycerol (500ml)
+#50% glycerol (500 ml)
+#LB medium (liquid) (3 L);
+->20 g of LB in every 1 L of distilled water. Autoclaved medium
+->in 1 of these L, 14 g of agar were added for making LB solid medium
+===plating E.coli===
+*Colonies of Top 10 ''E.coli'' were streaked on two plates
+*Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)
+Navigate to the [[Team:DTU-Denmark/Notebook/5_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/9_June_2013|Next]] Entry
+{{:Team:DTU-Denmark/Templates/EndPage}}