"
Team:DTU-Denmark/Notebook/7 June 2013
From 2013.igem.org
(Difference between revisions)
(→Procedure) |
|||
| (13 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{:Team:DTU-Denmark/Templates/StartPage|07 June 2013}} | {{:Team:DTU-Denmark/Templates/StartPage|07 June 2013}} | ||
| - | |||
=lab 208= | =lab 208= | ||
| Line 9: | Line 8: | ||
*prepare solutions | *prepare solutions | ||
*[[Team:DTU-Denmark/Methods/Autoclaving|autoclaving]] | *[[Team:DTU-Denmark/Methods/Autoclaving|autoclaving]] | ||
| + | *plate ''E.coli'' | ||
==Who was in the lab== | ==Who was in the lab== | ||
| Line 19: | Line 19: | ||
#50% glycerol (500 ml) | #50% glycerol (500 ml) | ||
#LB medium (liquid) (3 L); | #LB medium (liquid) (3 L); | ||
| - | 20 g of LB in every 1 L of distilled water. Autoclaved medium | + | ->20 g of LB in every 1 L of distilled water. Autoclaved medium |
| - | + | ||
| + | ->in 1 of these L, 14 g of agar were added for making LB solid medium | ||
| + | |||
| + | ===plating E.coli=== | ||
| + | *Colonies of Top 10 ''E.coli'' were streaked on two plates | ||
| + | *Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan) | ||
Navigate to the [[Team:DTU-Denmark/Notebook/5_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/9_June_2013|Next]] Entry | Navigate to the [[Team:DTU-Denmark/Notebook/5_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/9_June_2013|Next]] Entry | ||
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Latest revision as of 20:59, 16 September 2013
07 June 2013
Contents |
lab 208
Main purposes today
- helloworld-project
- prepare solutions
- autoclaving
- plate E.coli
Who was in the lab
Kristian
Procedure
- 1M CaCl_2 (200ml) weighed off 22.206 g CaCl_2
- 50% glycerol (500 ml)
- LB medium (liquid) (3 L);
->20 g of LB in every 1 L of distilled water. Autoclaved medium
->in 1 of these L, 14 g of agar were added for making LB solid medium
plating E.coli
- Colonies of Top 10 E.coli were streaked on two plates
- Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)
Navigate to the Previous or the Next Entry
