lab 208

Main purposes today

• helloworld-project
• prepare solutions
• autoclaving
• plate E.coli

Who was in the lab

Kristian

Procedure

1. 1M CaCl_2 (200ml) weighed off 22.206 g CaCl_2
2. 50% glycerol (500 ml)
3. LB medium (liquid) (3 L);

->20 g of LB in every 1 L of distilled water. Autoclaved medium

->in 1 of these L, 14 g of agar were added for making LB solid medium

plating E.coli

• Colonies of Top 10 E.coli were streaked on two plates
• Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)

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