Team:DTU-Denmark/Notebook/19 June 2013

From 2013.igem.org

(Difference between revisions)
 
(11 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:DTU-Denmark/Templates/StartPage|}}
+
{{:Team:DTU-Denmark/Templates/StartPage|19 June 2013}}
 +
 
 +
__TOC__
=208=
=208=
 +
<hr/>
 +
==Transformations==
 +
*from plate 3: [http://parts.igem.org/Part:BBa_I0500 I0500]
 +
*from plate 3: [http://parts.igem.org/Part:BBa_K808000 K808000]
 +
*from plate 3: [http://parts.igem.org/Part:BBa_K206001 K206001]
 +
*from plate 3: [http://parts.igem.org/Part:BBa_K564012 K564012]
 +
*from plate 3: [http://parts.igem.org/Part:BBa_I13458 I13458]
 +
*from plate 5: [http://parts.igem.org/Part:BBa_I13458 I13458]
-
==PCR of pZE21==
+
==PCR==
-
Mastermix:
+
-
*dNTP 5ul
+
-
*HF Buffer 50ul
+
-
*polymerase 2.5ul
+
-
*water 157.5ul
+
-
*fw primer (1A) 15ul
+
-
*rv primer 1B 15ul
+
-
*temlate 1ul/tube
+
-
 
+
-
 
+
-
 
+
 +
to amplify backbone pZE21
Navigate to the [[Team:DTU-Denmark/Notebook/18_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/20_June_2013|Next]] Entry
Navigate to the [[Team:DTU-Denmark/Notebook/18_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/20_June_2013|Next]] Entry
{{:Team:DTU-Denmark/Templates/EndPage}}
{{:Team:DTU-Denmark/Templates/EndPage}}

Latest revision as of 21:02, 16 September 2013

19 June 2013

Contents

208

Transformations

  • from plate 3: [http://parts.igem.org/Part:BBa_I0500 I0500]
  • from plate 3: [http://parts.igem.org/Part:BBa_K808000 K808000]
  • from plate 3: [http://parts.igem.org/Part:BBa_K206001 K206001]
  • from plate 3: [http://parts.igem.org/Part:BBa_K564012 K564012]
  • from plate 3: [http://parts.igem.org/Part:BBa_I13458 I13458]
  • from plate 5: [http://parts.igem.org/Part:BBa_I13458 I13458]

PCR

to amplify backbone pZE21

Navigate to the Previous or the Next Entry

Retrieved from "http://2013.igem.org/Team:DTU-Denmark/Notebook/19_June_2013"