Template:Kyoto/Notebook/Aug 23

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===Restriction Enzyme Digestion===
===Restriction Enzyme Digestion===
 +
<div class="experiment">
 +
<span class="author">Nakamoto and Kojima</span>
 +
{| class="wikitable"
 +
!Fusion6 antisense (1)||EcoRI||SpeI||buffer B||BSA||MilliQ||total
 +
|-
 +
|3||0.5||0.5||3||0.3||22.7||30
 +
|-
 +
|0.6||0.1||0||1||0.1||8.2||10
 +
|-
 +
|0.6||0||0.1||1||0.1||8.2||10
 +
|-
 +
|0.6||0||0||1||0.1||8.3||10
 +
|}
 +
{| class="wikitable"
 +
!Fusion6 antisense (1)||XbaI||PstI||buffer D||BSA||MilliQ||total
 +
|-
 +
|3||0.5||0.5||3||0.3||22.7||30
 +
|-
 +
|0.6||0.1||0||1||0.1||8.2||10
 +
|-
 +
|0.6||0||0.1||1||0.1||8.2||10
 +
|-
 +
|0.6||0||0||1||0.1||8.3||10
 +
|}
 +
{| class="wikitable"
 +
!Fusion1 antisense (1)||EcoRI||SpeI||buffer B||BSA||MilliQ||total
 +
|-
 +
|3.3||0.5||0.5||3||0.3||22.4||30
 +
|-
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|0.7||0.1||0||1||0.1||8.1||10
 +
|-
 +
|0.7||0||0.1||1||0.1||8.1||10
 +
|-
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|0.7||0||0||1||0.1||8.2||10
 +
|}
 +
{| class="wikitable"
 +
!pT181 antisense (1)||EcoRI||SpeI||buffer B||BSA||MilliQ||total
 +
|-
 +
|5.6||0.5||0.5||3||0.3||20.1||30
 +
|-
 +
|1.1||0.1||0||1||0.1||7.7||10
 +
|-
 +
|1.1||0||0.1||1||0.1||7.7||10
 +
|-
 +
|1.1||0||0||1||0.1||7.8||10
 +
|}
 +
{| class="wikitable"
 +
!pT181 antisense (1)||XbaI||PstI||buffer D||BSA||MilliQ||total
 +
|-
 +
|5.6||0.5||0.5||3||0.3||20.1||30
 +
|-
 +
|1.1||0.1||0||1||0.1||7.7||10
 +
|-
 +
|1.1||0||0.1||1||0.1||7.7||10
 +
|-
 +
|1.1||0||0||1||0.1||7.8||10
 +
|}
 +
*incubate at 37&deg;C from 17:29.
 +
</div>
===Electrophoresis===
===Electrophoresis===
===Gel Extraction===
===Gel Extraction===

Revision as of 01:18, 17 September 2013

Contents

Aug 23

Electrophoresis

No name

LaneSampleEnzyme1Enzyme2
1pT181 antisenseEcoRISpeI
2pT181 antisenseEcoRI--
3pT181 antisense--SpeI
4pT181 antisense----
5pT181 antisenseXbaIPstI
6pT181 antisenseXbaI--
7pT181 antisense--PstI
8pT181 antisense----
9100bp ladder----
10Fusion1 attenuatorEcoRISpeI
11Fusion1 attenuatorEcoRI--
12Fusion1 attenuator--SpeI
13Fusion1 attenuator----
14Fusion1 attenuatorXbaIPstI
15Fusion1 attenuatorXbaI--
16Fusion1 attenuator--PstI
17Fusion1 attenuator----

File:Igku xxxxxx.xxx

LaneSampleEnzyme1Enzyme2
1Fusion3m2 attenuatorEcoRISpeI
2Fusion3m2 attenuatorEcoRI--
3Fusion3m2 attenuator--SpeI
4Fusion3m2 attenuator----
5Fusion3m2 attenuatorXbaIPstI
6Fusion3m2 attenuatorXbaI--
7Fusion3m2 attenuator--PstI
8Fusion3m2 attenuator----
9100bp ladder----
10tetR aptamer 12_1MEcoRISpeI
11tetR aptamer 12_1MEcoRI--
12tetR aptamer 12_1M--SpeI
13tetR aptamer 12_1M----
14tetR aptamer 12_PEcoRISpeI
15tetR aptamer 12_PEcoRI--
16tetR aptamer 12_P--SpeI
17tetR aptamer 12_P----

File:Igku xxxxxx.xxx
Kojima and Honda

LaneSampleEnzyme1Enzyme2
1PlacSpeIPstI
2PlacSpeI--
3Plac--PstI
4Plac----
5SpinachEcoRISpeI
6SpinachEcoRI--
7Spinach--SpeI
8Spinach----
9100bp ladder----
10J23100SpeIPstI
11J23100SpeI--
12J23100--PstI
13J23100----
14tetR aptamer 12_1REcoRISpeI
15tetR aptamer 12_1REcoRI--
16tetR aptamer 12_1R--SpeI
17tetR aptamer 12_1R----

File:Igku xxxxxx.xxx

Restriction Enzyme Digestion

Nakamoto and Kojima

Fusion6 antisense (1)EcoRISpeIbuffer BBSAMilliQtotal
30.50.530.322.730
0.60.1010.18.210
0.600.110.18.210
0.60010.18.310
Fusion6 antisense (1)XbaIPstIbuffer DBSAMilliQtotal
30.50.530.322.730
0.60.1010.18.210
0.600.110.18.210
0.60010.18.310
Fusion1 antisense (1)EcoRISpeIbuffer BBSAMilliQtotal
3.30.50.530.322.430
0.70.1010.18.110
0.700.110.18.110
0.70010.18.210
pT181 antisense (1)EcoRISpeIbuffer BBSAMilliQtotal
5.60.50.530.320.130
1.10.1010.17.710
1.100.110.17.710
1.10010.17.810
pT181 antisense (1)XbaIPstIbuffer DBSAMilliQtotal
5.60.50.530.320.130
1.10.1010.17.710
1.100.110.17.710
1.10010.17.810
  • incubate at 37°C from 17:29.

Electrophoresis

Gel Extraction

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