"
Team:BYU Provo/Notebook/LargePhage/Springexp/Period4/Dailylog
From 2013.igem.org
(Difference between revisions)
Brymerr921 (Talk | contribs) (→6/10/13) |
|||
| Line 3: | Line 3: | ||
===6/10/13=== | ===6/10/13=== | ||
| + | Today we looked at a paper describing efficient ways to propagate large phage. They suggested agarose instead of agar, and at a concentration of 0.15% which is 1.5 g/L. Dr. Grose explained to us that agarose is more stable and forms larger pores, requiring a smaller amount of powder to produce a solid, stable gel. LB Agarose top agar can be plated onto normal LB bottom agar. We will make up some agarose top agar and see what size of plaques we get. | ||
===6/12/13=== | ===6/12/13=== | ||
Revision as of 20:45, 10 June 2013
Contents |
June
6/10/13
Today we looked at a paper describing efficient ways to propagate large phage. They suggested agarose instead of agar, and at a concentration of 0.15% which is 1.5 g/L. Dr. Grose explained to us that agarose is more stable and forms larger pores, requiring a smaller amount of powder to produce a solid, stable gel. LB Agarose top agar can be plated onto normal LB bottom agar. We will make up some agarose top agar and see what size of plaques we get.
6/12/13
6/14/13
6/17/13
6/19/13
6/21/13
