Template:Kyoto/Notebook/Aug 23

From 2013.igem.org

(Difference between revisions)

Revision as of 15:30, 17 September 2013

Aug 23

Electrophoresis

No name

Lane	Sample	Enzyme1	Enzyme2
1	pT181 antisense	EcoRI	SpeI
2	pT181 antisense	EcoRI	--
3	pT181 antisense	--	SpeI
4	pT181 antisense	--	--
5	pT181 antisense	XbaI	PstI
6	pT181 antisense	XbaI	--
7	pT181 antisense	--	PstI
8	pT181 antisense	--	--
9	100bp ladder	--	--
10	Fusion1 attenuator	EcoRI	SpeI
11	Fusion1 attenuator	EcoRI	--
12	Fusion1 attenuator	--	SpeI
13	Fusion1 attenuator	--	--
14	Fusion1 attenuator	XbaI	PstI
15	Fusion1 attenuator	XbaI	--
16	Fusion1 attenuator	--	PstI
17	Fusion1 attenuator	--	--

File:Igku xxxxxx.xxx

Lane	Sample	Enzyme1	Enzyme2
1	Fusion3m2 attenuator	EcoRI	SpeI
2	Fusion3m2 attenuator	EcoRI	--
3	Fusion3m2 attenuator	--	SpeI
4	Fusion3m2 attenuator	--	--
5	Fusion3m2 attenuator	XbaI	PstI
6	Fusion3m2 attenuator	XbaI	--
7	Fusion3m2 attenuator	--	PstI
8	Fusion3m2 attenuator	--	--
9	100bp ladder	--	--
10	tetR aptamer 12_1M	EcoRI	SpeI
11	tetR aptamer 12_1M	EcoRI	--
12	tetR aptamer 12_1M	--	SpeI
13	tetR aptamer 12_1M	--	--
14	tetR aptamer 12_P	EcoRI	SpeI
15	tetR aptamer 12_P	EcoRI	--
16	tetR aptamer 12_P	--	SpeI
17	tetR aptamer 12_P	--	--

File:Igku xxxxxx.xxx
Kojima and Honda

Lane	Sample	Enzyme1	Enzyme2
1	Plac	SpeI	PstI
2	Plac	SpeI	--
3	Plac	--	PstI
4	Plac	--	--
5	Spinach	EcoRI	SpeI
6	Spinach	EcoRI	--
7	Spinach	--	SpeI
8	Spinach	--	--
9	100bp ladder	--	--
10	J23100	SpeI	PstI
11	J23100	SpeI	--
12	J23100	--	PstI
13	J23100	--	--
14	tetR aptamer 12_1R	EcoRI	SpeI
15	tetR aptamer 12_1R	EcoRI	--
16	tetR aptamer 12_1R	--	SpeI
17	tetR aptamer 12_1R	--	--

File:Igku xxxxxx.xxx

Restriction Enzyme Digestion

Nakamoto and Kojima

Fusion6 antisense (1)	EcoRI	SpeI	buffer B	BSA	MilliQ	total
3	0.5	0.5	3	0.3	22.7	30
0.6	0.1	0	1	0.1	8.2	10
0.6	0	0.1	1	0.1	8.2	10
0.6	0	0	1	0.1	8.3	10

Fusion6 antisense (1)	XbaI	PstI	buffer D	BSA	MilliQ	total
3	0.5	0.5	3	0.3	22.7	30
0.6	0.1	0	1	0.1	8.2	10
0.6	0	0.1	1	0.1	8.2	10
0.6	0	0	1	0.1	8.3	10

Fusion1 antisense (1)	EcoRI	SpeI	buffer B	BSA	MilliQ	total
3.3	0.5	0.5	3	0.3	22.4	30
0.7	0.1	0	1	0.1	8.1	10
0.7	0	0.1	1	0.1	8.1	10
0.7	0	0	1	0.1	8.2	10

pT181 antisense (1)	EcoRI	SpeI	buffer B	BSA	MilliQ	total
5.6	0.5	0.5	3	0.3	20.1	30
1.1	0.1	0	1	0.1	7.7	10
1.1	0	0.1	1	0.1	7.7	10
1.1	0	0	1	0.1	7.8	10

pT181 antisense (1)	XbaI	PstI	buffer D	BSA	MilliQ	total
5.6	0.5	0.5	3	0.3	20.1	30
1.1	0.1	0	1	0.1	7.7	10
1.1	0	0.1	1	0.1	7.7	10
1.1	0	0	1	0.1	7.8	10

incubate at 37°C from 17:29.

Electrophoresis

No name

Lane	Sample	Enzyme1	Enzyme2
1	100bp	--	--
2	pT181 attenuator	EcoRI	SpeI
3	pT181 attenuator	XbaI	PstI
4	Fusion3m2 attenuator	EcoRI	SpeI
5	Fusion1 attenuator	XbaI	PstI
6	Fusion1 attenuator	EcoRI	SpeI
7	Fusion3m2 attenuator	XbaI	PstI
8	pT181 antisense	EcoRI	SpeI
9	pT181 antisense	XbaI	PstI
10	Fusion1 antisense	EcoRI	SpeI
11	Fusion1 antisense	XbaI	PstI
12	Fusion6 antisense	EcoRI	SpeI
13	Fusion6 antisense	XbaI	PstI
14	tetR aptamer 12_1M	EcoRI	SpeI
15	tetR aptamer 12_P	EcoRI	SpeI
16	tetR aptamer 12_1R	EcoRI	SpeI
17	100bp	--	--

File:Igku xxxxxx.xxx

Lane	Sample	Enzyme1	Enzyme2
1	100bp	--	--
2	Spinach	EcoRI	SpeI
3	J23100	SpeI	PstI
4	Plac	SpeI	PstI
5	100bp	--	--

File:Igku xxxxxx.xxx

Lane	Sample	Enzyme1	Enzyme2
1	100bp	--	--
2	Fusion6 antisense	EcoRI	SpeI
3	Fusion6 antisense	EcoRI	--
4	Fusion6 antisense	--	SpeI
5	Fusion6 antisense	--	--
6	pT181 antisense	XbaI	PstI
8	100bp	--	--
9	100bp	--	--
10	Fusion6 antisense	XbaI	PstI
11	Fusion6 antisense	XbaI	--
12	Fusion6 antisense	--	PstI
13	Fusion6 antisense	--	--
14	pT181 antisense	XbaI	--
16	100bp	--	--

File:Igku xxxxxx.xxx

Lane	Sample	Enzyme1	Enzyme2
1	100bp	--	--
2	Fusion1 antisense	EcoRI	SpeI
3	Fusion1 antisense	EcoRI	--
4	Fusion1 antisense	--	SpeI
5	Fusion1 antisense	--	--
6	pT181 antisense	--	PstI
8	100bp	--	--
9	100bp	--	--
10	pT181 antisense	EcoRI	SpeI
11	pT181 antisense	EcoRI	--
12	pT181 antisense	--	SpeI
13	pT181 antisense	--	--
14	pT181 antisense	--	--
16	100bp	--	--

File:Igku xxxxxx.xxx

Gel Extraction

Nakamoto, Ashida, Kojima, Okazaki & Matsumoto

Lane	DNA	Enzyme
1	100bp ladder	--
2	pT181 attenuator	XbaI & PstI
3	pT181 attenuator	XbaI & PstI
5	Fusion3m2 attenuator	EcoRI & SpeI
6	Fusion3m2 attenuator	EcoRI & SpeI
8	Fusion1 attenuator	XbaI & PstI
9	Fusion1 attenuator	XbaI & PstI
11	Fusion1 attenuator	EcoRI & SpeI
12	Fusion1 attenuator	EcoRI & SpeI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Lane	DNA	Enzyme
1	100bp ladder	--
2	Fusion3m2 attenuator	XbaI & PstI
3	Fusion3m2 attenuator	XbaI & PstI
5	pT181 antisense	EcoRI & SpeI
6	pT181 antisense	EcoRI & SpeI
8	pT181 antisense	XbaI & PstI
9	pT181 antisense	XbaI & PstI
11	Fusion1 antisense	XbaI & PstI
12	Fusion1 antisense	XbaI & PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Lane	DNA	Enzyme
1	100bp ladder	--
2	Fusion6 attenuator	XbaI & PstI
3	Fusion6 attenuator	XbaI & PstI
5	tetR aptamer 12_1M	EcoRI & SpeI
6	tetR aptamer 12_1M	EcoRI & SpeI

Lane	DNA	Enzyme
1	100bp ladder	--
2	tetR aptamer 12_P	EcoRI & SpeI
3	tetR aptamer 12_P	EcoRI & SpeI
5	tetR aptamer 12_1R	EcoRI & SpeI
6	tetR aptamer 12_1R	EcoRI & SpeI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
Ashida

Lane	DNA	Enzyme
1	100bp ladder	--
2	Fusion6 antisense	EcoRI & SpeI
3	Fusion6 antisense	EcoRI & SpeI
5	Fusion6 attenuator	XbaI & PstI
6	Fusion6 attenuator	XbaI & PstI
8	Fusion1 antisense	EcoRI & SpeI
9	Fusion1 antisense	EcoRI & SpeI
11	pT181 antisense	EcoRI & SpeI
12	pT181 antisense	EcoRI & SpeI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Lane	DNA	Enzyme
1	100bp ladder	--
3	pT181 antisense	XbaI & PstI
4	pT181 antisense	XbaI & PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Lane	DNA	Enzyme
1	100bp ladder	--
3	Plac	SpeI & PstI
4	Plac	SpeI & PstI

Lane	DNA	Enzyme
1	100bp ladder	--
3	J23100	SpeI & PstI
5	Spinach	EcoRI & SpeI
6	Spinach	EcoRI & SpeI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

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 [[File:igku_xxbeforexx.xxx]]<br>
 [[File:igku_xxafterxx.xxx]]<br>
-{| class="wikitable"
-!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
-|-
-|1|| || || ||
-|-
-|（ΦωΦ^）|| || || ||
-|}
 {| class="wikitable"
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@@ Line 359: / Line 352: @@
 [[File:igku_xxbeforexx.xxx]]<br>
 [[File:igku_xxafterxx.xxx]]<br>
-{| class="wikitable"
-!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
-|-
-|1|| || || ||
-|-
-|（ΦωΦ^）|| || || ||
-|}
 {| class="wikitable"
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@@ Line 394: / Line 380: @@
 [[File:igku_xxbeforexx.xxx]]<br>
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-{| class="wikitable"
-!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
-|-
-|1|| || || ||
-|-
-|（ΦωΦ^）|| || || ||
-|}
 <span class="author">Ashida</span>
 {| class="wikitable"
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 [[File:igku_xxbeforexx.xxx]]<br>
 [[File:igku_xxafterxx.xxx]]<br>
-{| class="wikitable"
-!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
-|-
-|1|| || || ||
-|-
-|（ΦωΦ^）|| || || ||
-|}
 {| class="wikitable"
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 [[File:igku_xxbeforexx.xxx]]<br>
 [[File:igku_xxafterxx.xxx]]<br>
-{| class="wikitable"
-!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
-|-
-|1|| || || ||
-|-
-|（ΦωΦ^）|| || || ||
-|}
 {| class="wikitable"
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@@ Line 472: / Line 437: @@
 [[File:igku_xxbeforexx.xxx]]<br>
 [[File:igku_xxafterxx.xxx]]<br>
-{| class="wikitable"
-!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
-|-
-|1|| || || ||
-|-
-|（ΦωΦ^）|| || || ||
-|}
 </div>

Template:Kyoto/Notebook/Aug 23

From 2013.igem.org

Revision as of 15:30, 17 September 2013

Contents

Aug 23

Electrophoresis

Restriction Enzyme Digestion

Electrophoresis

Gel Extraction