Template:Team:Uppsala/JS/notebook

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ds = '<div id="dairy-text"><h1>Sunday 2013-07-07</h1> Name of participants:Anders Edlund (AE) <h2>Ongoing constructs</h2> 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 77. pSB4S15-C-resistance <h2>Todays work</h2> Transformation (AE) 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 77. pSB4S15-C-resistance </div>';

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{

ds = '<div id="dairy-text"><h1>Saturday 2013-07-06</h1> Name of participants: Stephanie Herman (SH), Anders Edlund (AE), Nafisa Bashir (NB) <h2>Ongoing constructs:</h2> E.coli (strain D5α): 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 <h2>Todays work</h2> Plasmid Preparation: 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 Transformation: 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 <h2>Results</h2> Previous day: The O/N on the nonplasmids 4, 10, 11, 12 from the previous day had grown at a previously unexperienced rate. A contamination of the original plates from Stefan Roos was suspected as the NC was clear. The O/N was put in the cold room awaiting examination by microscope. Today: Plasmid Preparation: 42. pSB4S15-red 61. pSB1C3-CP1: (1) 203.2, (2) 202.9 62. pSB1C3-CP8: (1) 254.5, (2) 229.2 63. pSB1C3-CP11: (1) 165.0, (2) 260.1 64. pSB1C3-CP29: (1) 315.4, (2) 221.9 65. pSB1C3-CP30: (1) 243.1, (2) 101.5 66. pSB1C3-CP41: (1) 190.7, (2) 68.4 67. pSB1C3-Cp44: (1) 169.1, (2) 188.7 </div>';

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ds = '<div id="dairy-text"><h1>Friday 2013-07-05</h1> Name of participants: Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A., Christoffer Ahlström (CA), Anton Berglund (AB), Stephanie Herman (SH), Mikael Strandgren (MS), Viktor Blomkvist (VB), Nafisa Bashir (NB), Magnus, Thorsteinn, Alexander, Nils, Christoffer <h2>Ongoing constructs:</h2> 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-CP44 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA L. reuteri Lb4. 100-23 no plasmid Lb12. DSM 20016 no plasmid L. plantarum: Lb10. 256 no plasmid Lb11. 36E no plasmid L. thermophilus: Lb19. L.thermophilus Todays work Screening PCR & gel analysis of constructs: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 61.1 pSB1C3-CP1 61.2 pSB1C3-CP1 62.1 pSB1C3-CP8 62.2 pSB1C3-CP8 62.3 pSB1C3-CP8 62.4 pSB1C3-CP8 63.1 pSB1C3-CP11 63.2 pSB1C3-CP11 63.3 pSB1C3-CP11 63.4 pSB1C3-CP11 64.1.1 pSB1C3-CP29 64.1.2 pSB1C3-CP29 64.1.3 pSB1C3-CP29 64.1.4 pSB1C3-CP29 65.1.1 pSB1C3-CP30 65.1.2 pSB1C3-CP30 65.2.1 pSB1C3-CP30 65.2.2 pSB1C3-CP30 66.5 pSB1C3-CP41 66.6 pSB1C3-CP41 66.7 pSB1C3-CP41 66.8 pSB1C3-CP41 67.1.1 pSB1C3-CP44 67.1.2 pSB1C3-CP44 67.2.1 pSB1C3-CP44 67.2.2 pSB1C3-CP44 Plasmid prep & glycerol stock: 48. pSB1C3-B0034-His-STS O/N: 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 47.(9, 20) pSB1C3-B0034-STS Lb4. reuteri 100-23 no plasmid Lb10. plantarum 256 no plasmid Lb11. plantarum 36E no plasmid Lb12. reuteri DSM 20016 no plasmid Re-streaks: Lb19. L.thermophilus <h2>Results</h2> The O/N on 42. pSB4S15-red was done improperly in 15 ml falcon tubes and the lids were screwed on too tightly. A new O/N was done on 42. pSB4S15-red by NB. PCR screen and gel electrophoresis: 61.1 pSB1C3-CP1 61.2 pSB1C3-CP1 62.1 pSB1C3-CP8 62.2 pSB1C3-CP8 62.3 pSB1C3-CP8 62.4 pSB1C3-CP8 63.1 pSB1C3-CP11 63.2 pSB1C3-CP11 63.3 pSB1C3-CP11 63.4 pSB1C3-CP11 64.1.1 pSB1C3-CP29 64.1.2 pSB1C3-CP29 64.1.3 pSB1C3-CP29 64.1.4 pSB1C3-CP29 65.1.1 pSB1C3-CP30 65.1.2 pSB1C3-CP30 65.2.1 pSB1C3-CP30 65.2.2 pSB1C3-CP30 66.5 pSB1C3-CP41 66.6 pSB1C3-CP41 66.7 pSB1C3-CP41 66.8 pSB1C3-CP41 67.1.1 pSB1C3-CP44 67.1.2 pSB1C3-CP44 67.2.1 pSB1C3-CP44 67.2.2 pSB1C3-CP44 *The gel only showed smudgy bands. succesful 47.9 pSB1C3-B0034-STS 47.20 pSB1C3-B0034-STS failed 43. pSB1C3-B0034-TAL 46. pSB1C3-B0034-His-4CL No contamination in negative control -_- Religation from yesterday failed. No colonies, only a few at 76, both red and white. <h2>ther experiments</h2> Contamination control: CA * *Contamination control of growth medium for Lc.lactis and Lb.reuteri. Two test from both bottles. E.coli growth test: CA * *To see if strain 1 of e.coli in strain database 13 grow on M17 and MRS plates as well. </div>';

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    ds = '<div id="dairy-text"><h1>Sunday 2013-07-07</h1><br><b>Name of participants:</b>Anders Edlund (AE)<br><br><h2>Ongoing constructs</h2><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>77. pSB4S15-C-resistance<br><br><h2>Todays work</h2><br><b>Transformation (AE)</b><br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>77. pSB4S15-C-resistance<br></div>';
   }
-  else if(id == 'd2013716')
+  else if(id == 'd201376')
   {
    ds = '<div id="dairy-text"><h1>Saturday 2013-07-06</h1><br><b>Name of participants:</b> Stephanie Herman (SH), Anders Edlund (AE), Nafisa Bashir (NB)<br><br><h2>Ongoing constructs:</h2><br><b>E.coli (strain D5α):</b><br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br><h2>Todays work</h2><br><b>Plasmid Preparation:</b> <br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br><br><b>Transformation: </b><br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br><h2>Results</h2><br><b>Previous day:</b><br>The O/N on the nonplasmids 4, 10, 11, 12 from the previous day had grown at a previously unexperienced rate. A contamination of the original plates from Stefan Roos was suspected as the NC was clear. The O/N was put in the cold room awaiting examination by microscope.<br><br><b>Today:</b><br><b>Plasmid Preparation:</b><br>42. pSB4S15-red<br>61. pSB1C3-CP1: (1) 203.2, (2) 202.9<br>62. pSB1C3-CP8: (1) 254.5, (2) 229.2<br>63. pSB1C3-CP11: (1) 165.0, (2) 260.1<br>64. pSB1C3-CP29: (1) 315.4, (2) 221.9<br>65. pSB1C3-CP30: (1) 243.1, (2) 101.5<br>66. pSB1C3-CP41: (1) 190.7, (2) 68.4<br>67. pSB1C3-Cp44: (1) 169.1, (2) 188.7<br></div>';
   }
-  else if(id == 'd2013715')
+  else if(id == 'd201375')
   {
    ds = '<div id="dairy-text"><h1>Friday 2013-07-05</h1><br><b>Name of participants:</b> Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A., Christoffer Ahlström (CA), Anton Berglund (AB), Stephanie Herman (SH), Mikael Strandgren (MS), Viktor Blomkvist (VB), Nafisa Bashir (NB), Magnus, Thorsteinn, Alexander, Nils, Christoffer<br><br><h2>Ongoing constructs:</h2><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-CP44<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br><br><b>L. reuteri</b><br>Lb4. 100-23 no plasmid<br>Lb12. DSM 20016 no plasmid<br><br><b>L. plantarum:</b><br>Lb10. 256 no plasmid<br>Lb11. 36E no plasmid<br><br><b>L. thermophilus:</b><br>Lb19. L.thermophilus<br><br><b>Todays work</b><br>Screening PCR & gel analysis of constructs:<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>61.1 pSB1C3-CP1<br>61.2 pSB1C3-CP1<br>62.1 pSB1C3-CP8<br>62.2 pSB1C3-CP8<br>62.3 pSB1C3-CP8<br>62.4 pSB1C3-CP8<br>63.1 pSB1C3-CP11<br>63.2 pSB1C3-CP11<br>63.3 pSB1C3-CP11<br>63.4 pSB1C3-CP11<br>64.1.1 pSB1C3-CP29<br>64.1.2 pSB1C3-CP29<br>64.1.3 pSB1C3-CP29<br>64.1.4 pSB1C3-CP29<br>65.1.1 pSB1C3-CP30<br>65.1.2 pSB1C3-CP30<br>65.2.1 pSB1C3-CP30<br>65.2.2 pSB1C3-CP30<br>66.5 pSB1C3-CP41<br>66.6 pSB1C3-CP41<br>66.7 pSB1C3-CP41<br>66.8 pSB1C3-CP41<br>67.1.1 pSB1C3-CP44<br>67.1.2 pSB1C3-CP44<br>67.2.1 pSB1C3-CP44<br>67.2.2 pSB1C3-CP44<br><br><b>Plasmid prep & glycerol stock:</b><br>48. pSB1C3-B0034-His-STS<br><br><b>O/N:</b> <br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>47.(9, 20) pSB1C3-B0034-STS<br>Lb4. reuteri 100-23 no plasmid<br>Lb10. plantarum 256 no plasmid<br>Lb11. plantarum 36E no plasmid<br>Lb12. reuteri DSM 20016 no plasmid<br><br><b>Re-streaks:</b><br>Lb19. L.thermophilus<br><br><h2>Results</h2><br>The O/N on 42. pSB4S15-red was done improperly in 15 ml falcon tubes and the lids were screwed on too tightly. A new O/N was done on 42. pSB4S15-red by NB.<br><br><b>PCR screen and gel electrophoresis:</b><br>61.1 pSB1C3-CP1<br>61.2 pSB1C3-CP1<br>62.1 pSB1C3-CP8<br>62.2 pSB1C3-CP8<br>62.3 pSB1C3-CP8<br>62.4 pSB1C3-CP8<br>63.1 pSB1C3-CP11<br>63.2 pSB1C3-CP11<br>63.3 pSB1C3-CP11<br>63.4 pSB1C3-CP11<br>64.1.1 pSB1C3-CP29<br>64.1.2 pSB1C3-CP29<br>64.1.3 pSB1C3-CP29<br>64.1.4 pSB1C3-CP29<br>65.1.1 pSB1C3-CP30<br>65.1.2 pSB1C3-CP30<br>65.2.1 pSB1C3-CP30<br>65.2.2 pSB1C3-CP30<br>66.5 pSB1C3-CP41<br>66.6 pSB1C3-CP41<br>66.7 pSB1C3-CP41<br>66.8 pSB1C3-CP41<br>67.1.1 pSB1C3-CP44<br>67.1.2 pSB1C3-CP44<br>67.2.1 pSB1C3-CP44<br>67.2.2 pSB1C3-CP44<br><br>*The gel only showed smudgy bands.<br><br><b>succesful</b><br>47.9 pSB1C3-B0034-STS<br>47.20 pSB1C3-B0034-STS<br><br><b>failed</b><br>43. pSB1C3-B0034-TAL<br>46. pSB1C3-B0034-His-4CL<br><br>No contamination in negative control -_-<br>Religation from yesterday failed. No colonies, only a few at 76, both red and white. <br><br><h2>ther experiments</h2><br><b>Contamination control:</b> CA *<br><br><i>*Contamination control of growth medium for Lc.lactis and Lb.reuteri.<br> Two test from both bottles. </i><br><br><b>E.coli growth test:</b> CA *<br><br><i>*To see if strain 1 of e.coli in strain database 13 grow on M17 and MRS plates as well. </i></div>';

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