"
Team:Groningen/Project/secretion
From 2013.igem.org
(Difference between revisions)
| (12 intermediate revisions not shown) | |||
| Line 6: | Line 6: | ||
</p> | </p> | ||
<p> | <p> | ||
| - | We developed 12 different types of spider | + | We developed 12 different types of <a href="https://2013.igem.org/Team:Groningen/Navigation/Parts">spider silks</a> (figure 1). We have one spider silk protein with a N and C terminus, a spider silk without a N and C terminus and a spidersilk without one block (explain the difference). A codon optimization script was developed to codon optimize the spider silk for <i>b. subtilis</i>. |
Codon optimization (state why we did codon optimization and link towards the codon optimazation page on the wiki for all the details). Every silk gene was codon optimized twice with different results. All the codon optimized silk genes were ordered and made synthetically (figure 2). | Codon optimization (state why we did codon optimization and link towards the codon optimazation page on the wiki for all the details). Every silk gene was codon optimized twice with different results. All the codon optimized silk genes were ordered and made synthetically (figure 2). | ||
</p> | </p> | ||
| Line 13: | Line 13: | ||
| - | <div align=" | + | <div align="left"> |
| - | <table> | + | <table id="layout" width=40%> |
<tr> | <tr> | ||
<td> | <td> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/9/95/Masp2.jpg" width="100%"> |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> | <td> | ||
| - | <font size="1">Figure 1, | + | <font size="1">Figure 1, Major ampullate Spidroin 2 (MaSp2) from <i>Argiope aurantia</i></font> |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 32: | Line 32: | ||
<br> | <br> | ||
| - | <div align=" | + | <div align="left"> |
| - | <table> | + | <table id="layout" width=40%> |
<tr> | <tr> | ||
<td> | <td> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/7/78/Our_Silks.png" width=" | + | <img src="https://static.igem.org/mediawiki/2013/7/78/Our_Silks.png" width="100%" > |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> | <td> | ||
| - | <font size="1">Figure 2: | + | <font size="1">Figure 2, Our constructed silk. All <a href="https://2013.igem.org/Team:Groningen/Navigation/Parts">parts</a> are made into biobricks and can be combined together. </font> |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 57: | Line 57: | ||
<div align="left"> | <div align="left"> | ||
| - | <table> | + | <table id="layout" width=20%> |
<tr> | <tr> | ||
<td> | <td> | ||
| - | <img src="https://static.igem.org/mediawiki/igem.org/8/82/Sec_bsub.gif" width=" | + | <img src="https://static.igem.org/mediawiki/igem.org/8/82/Sec_bsub.gif" width="100%"> |
</td> | </td> | ||
</tr> | </tr> | ||
Latest revision as of 11:05, 18 September 2013
Silk production
We developed 12 different types of spider silks (figure 1). We have one spider silk protein with a N and C terminus, a spider silk without a N and C terminus and a spidersilk without one block (explain the difference). A codon optimization script was developed to codon optimize the spider silk for b. subtilis. Codon optimization (state why we did codon optimization and link towards the codon optimazation page on the wiki for all the details). Every silk gene was codon optimized twice with different results. All the codon optimized silk genes were ordered and made synthetically (figure 2).
|
| Figure 1, Major ampullate Spidroin 2 (MaSp2) from Argiope aurantia |
|
| Figure 2, Our constructed silk. All parts are made into biobricks and can be combined together. |
The silk genes are provided with an signal peptide and a strep tag. The signal peptide will ensure the secretion of silk by the natural pathways of b. subtilis (figure 3). The strep tag will be used for our coating mechanism.
|
| Figure 3: the secretion pathway of silk |
