Team:BYU Provo/Notebook/Phage Purification/Summerexp

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Latest revision as of 20:33, 18 September 2013

Phage Purification July - August Notebook
Phage Purification March-April May-June July-August September-October	<< Previous Next >> July 1 - July 15 We are working on getting Electron Microscope images of our purified phage. Daily log Experiment Listing
	July 16 - July 31 In these two weeks, we ran a CsCl gradient for the T4 group. We also discussed the children's book and other team projects. Daily log Experiment Listing
	August 1 - August 16 These two weeks were spent focussing on isolating T7 phage. After successful results coming from T4, we had high hopes of getting the same results with T7. Our first attempts were unsuccessful but we believe we will be able to isolate the mutant T7 in the following weeks. Daily log Experiment Listing
	August 17 - August 31 We ran into more problems attempting to purify the T7 phage. While we were at first able to get a clear band of phage using the cesium chloride gradient we were no longer able to achieve the same results. We focussed on creating a gradient that would allow the T7 phage to band once again but we did not have any luck in doing so. Daily log Experiment Listing << Previous Next >>

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-| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage March - April Notebook'''</font>
+| colspan="3" | <font color="#333399" size="5" font face="Calibri">
+: '''Phage Purification July - August Notebook'''</font>
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 <font color="#333399" size="3" font face="Calibri">
-: [[Team:BYU_Provo/Phage_Purification|Overview]]
+<font size = "4">
+: <u> '''Phage Purification''' </u> </font>
 : [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
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-<html>							<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog"><< Previous</a>
+<html>							<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp"><< Previous</a>
 							<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Fallexp" style="display:block;float:right;">Next >></a>
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-<font size="5" font face="Calibri"> '''March 15 - March 31''' </font>
+<font size="5" font face="Calibri"> '''July 1 - July 15''' </font>
 <br>
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 <font size="3" font face="Calibri">
-This marks the start of our small phage group! Although we are still on the background-research phase and not doing any wet-lab experiments, we are sure our work will be worthwhile. As Dr. Grose, our wonderful instructor, says "a month in the lab will say you a day in the library." After two weeks of hardcore literature search, we now have a clear idea of where our project is headed. And we are super excited to get started!
+We are working on getting Electron Microscope images of our purified phage.
 </font>
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 [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period8/Explist|Experiment Listing]]
-[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period8/PR|Presentation]] </font>
 <br>
-| style="width: 20%; background-color: transparent;"| [[File:Winter1.JPG|250px|center]]
+| style="width: 20%; background-color: transparent;"| [[File:manyphage.JPG|220px|center]]
 |-
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 | style="width: 18%; background-color: transparent;"|
-| <font size="5" font face="Calibri"> '''April 1 - April 14''' </font>
+| <font size="5" font face="Calibri"> '''July 16 - July 31''' </font>
 <br>
-<font size="3" font face="Calibri"> Add description of the two weeks </font>
+<font size="3" font face="Calibri"> In these two weeks, we ran a CsCl gradient for the T4 group. We also discussed the children's book and other team projects. </font>
 <br>
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 [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period9/Explist|Experiment Listing]]
-[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period9/PR| Progress Report]] </font>
 <br>
-| style="width: 20%; background-color: transparent;"| [[File:Winter2.JPG|250px|center]]
+| style="width: 20%; background-color: transparent;"| [[File:PhageCircle.JPG|220px|center]]
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 | style="width: 18%; background-color: transparent;"|
-| <font size="5" font face="Calibri"> '''April 15 - April 30''' </font>
+| <font size="5" font face="Calibri"> '''August 1 - August 16''' </font>
 <br>
-<font size="3" font face="Calibri"> Add description of the two weeks! </font>
+<font size="3" font face="Calibri"> These two weeks were spent focussing on isolating T7 phage.  After successful results coming from T4, we had high hopes of getting the same results with T7.  Our first attempts were unsuccessful but we believe we will be able to isolate the mutant T7 in the following weeks.   </font>
 <br>
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 [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period10/Explist|Experiment Listing]]
-[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period10/PR| Progress Report]] </font>
+<br>
+| style="width: 20%; background-color: transparent;"| [[File:singlephage.JPG|220px|center]]
+|-
+| style="width: 18%; background-color: transparent;"|
+| <font size="5" font face="Calibri"> '''August 17 - August 31''' </font>
+<br>
+<font size="3" font face="Calibri"> We ran into more problems attempting to purify the T7 phage.  While we were at first able to get a clear band of phage using the cesium chloride gradient we were no longer able to achieve the same results.  We focussed on creating a gradient that would allow the T7 phage to band once again but we did not have any luck in doing so.  </font>
 <br>
-<html>							<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period3/Dailylog"><< Previous</a>
+<font color="#333399" size="4" font face="Calibri">
+[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period11/Dailylog|Daily log]]
+[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period11/Explist|Experiment Listing]]
+<br>
+<html>							<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp"><< Previous</a>
 							<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Fallexp" style="display:block;float:right;">Next >></a>
 </html>
-| style="width: 20%; background-color: transparent;"| [[File:Winter3.JPG|250px|center]]
+| style="width: 20%; background-color: transparent;"| [[File:phage.JPG|220px|center]]