Team:BYU Provo/Notebook/LargePhage/Summerexp/Period2/Dailylog

From 2013.igem.org

(Difference between revisions)
(9/18/13)
(9/18/13)
Line 11: Line 11:
Fractions 18, 19, 17, 16, and 14 had large clearings still at 10^-2 while 13 and 12 just had individual plaques.
Fractions 18, 19, 17, 16, and 14 had large clearings still at 10^-2 while 13 and 12 just had individual plaques.
Fractions 18, 19, 17, 16, and 14 had individual plaques at 10^-4.
Fractions 18, 19, 17, 16, and 14 had individual plaques at 10^-4.
 +
 +
We also measured the capsids and tails of the TEM images.
===9/27/13===
===9/27/13===

Revision as of 21:16, 18 September 2013

Contents

September

9/16/13

Today Jade and LJ helped us spot test all of the fractions above the band from our last CsCl gradient. These fractions were numbered 12-19, but 15 was skipped. Each fraction was spot tested at a titer of 10^0, 10^-2, and 10^-4 and incubated agar side down at 37C overnight.

9/18/13

We looked at the results from the spot test today. We found that: All fractions had phage at 10^0 Fractions 18, 19, 17, 16, and 14 had large clearings still at 10^-2 while 13 and 12 just had individual plaques. Fractions 18, 19, 17, 16, and 14 had individual plaques at 10^-4.

We also measured the capsids and tails of the TEM images.

9/27/13

0