Team:BYU Provo/Notebook/LargePhage/Summerexp/Period2/Dailylog
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Fractions 18, 19, 17, 16, and 14 had large clearings still at 10^-2 while 13 and 12 just had individual plaques. | Fractions 18, 19, 17, 16, and 14 had large clearings still at 10^-2 while 13 and 12 just had individual plaques. | ||
Fractions 18, 19, 17, 16, and 14 had individual plaques at 10^-4. | Fractions 18, 19, 17, 16, and 14 had individual plaques at 10^-4. | ||
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+ | We also measured the capsids and tails of the TEM images. | ||
===9/27/13=== | ===9/27/13=== |
Revision as of 21:16, 18 September 2013
Contents |
September
9/16/13
Today Jade and LJ helped us spot test all of the fractions above the band from our last CsCl gradient. These fractions were numbered 12-19, but 15 was skipped. Each fraction was spot tested at a titer of 10^0, 10^-2, and 10^-4 and incubated agar side down at 37C overnight.
9/18/13
We looked at the results from the spot test today. We found that: All fractions had phage at 10^0 Fractions 18, 19, 17, 16, and 14 had large clearings still at 10^-2 while 13 and 12 just had individual plaques. Fractions 18, 19, 17, 16, and 14 had individual plaques at 10^-4.
We also measured the capsids and tails of the TEM images.
9/27/13