Template:Kyoto/Notebook/Aug 24

From 2013.igem.org

(Difference between revisions)
(Gel Extraction)
(Restriction Enzyme Digestion)
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===Restriction Enzyme Digestion===
===Restriction Enzyme Digestion===
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<!-- こっから -->
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<div class="experiment">
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<span class="author">No name</span>
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{| class="wikitable"
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!tetR aptamer 12_P(2)||EcoRI||SpeI||10x Buffer B||100x BSA||MilliQ||total
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|-
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|2.7&micro;L||0.5&micro;L||0.5&micro;L||3&micro;L||0.3&micro;L||23&micro;L||30&micro;L
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|-
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|0.3&micro;L||0&micro;L||0&micro;L||1&micro;L||0.1&micro;L||8.6&micro;L||10&micro;L
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|}
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{| class="wikitable"
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!tetR aptamer 12_1R(2)||EcoRI||SpeI||10x Buffer B||100x BSA||MilliQ||total
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|-
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|2.6&micro;L||0.5&micro;L||0.5&micro;L||3&micro;L||0.3&micro;L||23.1&micro;L||30&micro;L
 +
|-
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|0.3&micro;L||0&micro;L||0&micro;L||1&micro;L||0.1&micro;L||8.6&micro;L||10&micro;L
 +
|}
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{| class="wikitable"
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!tetR aptamer 12_M(1)||EcoRI||SpeI||10x Buffer B||100x BSA||MilliQ||total
 +
|-
 +
|2.7&micro;L||0.5&micro;L||0.5&micro;L||3&micro;L||0.3&micro;L||23&micro;L||30&micro;L
 +
|-
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|0.3&micro;L||0&micro;L||0&micro;L||1&micro;L||0.1&micro;L||8.6&micro;L||10&micro;L
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|}
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===Master Plate===
===Master Plate===
===Electrophoresis===
===Electrophoresis===

Revision as of 01:43, 19 September 2013

Contents

Aug 24

Gel Extraction

Ashida

Nameconcentration[µg/mL]260/280260/230
8/22 aptamer 12_P (EcoRI & SpeI)8821.3--
8/22 aptamer 12_1R (EcoRI & SpeI)6323.1--
8/22 aptamer 12_1M (EcoRI & SpeI)4022.0--
8/23 Fusion6 antisense (EcoRI & SpeI)6.01.600.17
8/23 Fusion6 antisense (XbaI & PstI)6.01.710.25
8/23 pT181 antisense (EcoRI & SpeI)6.02.220.15
8/23 pT181 antisense (XbaI & PstI)6.02.100.04
8/22 Plac (SpeI & PstI)6.01.470.09
8/22 Fusion1 antisense (EcoRI & SpeI)102.160.01
8/22 J23100 (SpeI & PstI)6.01.520.35
Nameconcentration[µg/mL]260/280260/230
8/22 Spinach (EcoRI & SpeI)8.01.150.77
8/22 Fusion6 antisense (XbaI & PstI)441.070.96
8/22 Fusion1 antisense (XbaI & PstI)2--1.00
8/22 pT181 antisense (XbaI & PstI)3.2.010.40
8/22 pT181 antisense (EcoRI & SpeI)81.130.82
8/22 Fusion3m2 attenuator (XbaI & PstI)52.2771.50
8/22 Fusion3m2 attenuator (EcoRI & SpeI)341.161.02
8/22 Fusion1 attenuator (XbaI & PstI)141.400.62
8/22 Fusion1 attenuator (EcoRI & SpeI)161.270.61
8/22 pT181 attenuator (XbaI & PstI)5--0.88
  • These samples were performed ethanol ethanl precipitation.

Restriction Enzyme Digestion

No name

tetR aptamer 12_P(2)EcoRISpeI10x Buffer B100x BSAMilliQtotal
2.7µL0.5µL0.5µL3µL0.3µL23µL30µL
0.3µL0µL0µL1µL0.1µL8.6µL10µL
tetR aptamer 12_1R(2)EcoRISpeI10x Buffer B100x BSAMilliQtotal
2.6µL0.5µL0.5µL3µL0.3µL23.1µL30µL
0.3µL0µL0µL1µL0.1µL8.6µL10µL
tetR aptamer 12_M(1)EcoRISpeI10x Buffer B100x BSAMilliQtotal
2.7µL0.5µL0.5µL3µL0.3µL23µL30µL
0.3µL0µL0µL1µL0.1µL8.6µL10µL

Master Plate

===Electrophoresis===