Aromatics Scouts

A FAST, EASY AND ACCURATE METHOD TO DETECT TOXIC AROMATIC COMPOUNDS

Aromatic pollution is becoming a worldwide concern. Monitoring aromatics pollution, however, remains a substantial challenge. Noting the abundant genomic data of prokaryotes from aromatics-rich environment, Peking iGEM applied part mining to the genetic repertoire to develop a comprehensive set of transcriptional-regulator-based biosensors for aromatics. The transcription regulators for each typical class of organic compounds were first bioinformatically determined and then promoter engineering and protein engineering were performed to tune their properties functionally.
To expand the detection range, enzymes in upper pathways, working as plug-ins, were coupled with existing biosensors to degrade aromatics to detectable compounds. All these sensors are capable of detect a group of aromatics, and multi-sensor assay may provide an insight of detect certain components in samples. Additionally, for in situ detection, we construct the band pass filter to detect a certain range of concentration. Responses of biosensors equipped with band-pass filter can robustly reflect the concentration of environmental samples.
In conclusion, Peking iGEM has remarkably enriched the library of biosensors for aromatics and enabled quantitative in situ detection for environmental monitoring. These biosensors, we expect, will be also potent for metabolic engineering and well-characterized synthetic biological tools.