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Team:Grenoble-EMSE-LSU/Documentation/Biobricks
From 2013.igem.org
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<p>Below is a comparison of the 3D structures of monomeric dsRed and KillerRed:<br><br> | <p>Below is a comparison of the 3D structures of monomeric dsRed and KillerRed:<br><br> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/8/8c/DsRed_alongside_KillerRed.png" alt="dsRed and KillerRed protein structures. Credits to Carpentier P., Violot S., Blanchoin L., Bourgeois D. for KillerRed, and Strongin D.E., Bevis B., Khuong N., Downing M.E., Strack R.L., Sundaram K., Glick B.S., Keenan R.J. for the dsRed structure.">< | + | <p align="center"><img src="https://static.igem.org/mediawiki/2013/8/8c/DsRed_alongside_KillerRed.png" alt="dsRed and KillerRed protein structures."></p><br> |
| + | <p id="legend"><strong><em>A comparison of the 3D structures of monomerix dsRed (left) and dimeric KillerRed (right)</em></strong><br> | ||
| + | <em><strong>Credits to Carpentier P., Violot S., Blanchoin L., Bourgeois D. for the KillerRed structure, and Strongin D.E., Bevis B., Khuong N., Downing M.E., Strack R.L., Sundaram K., Glick B.S., Keenan R.J. for the dsRed structure.</strong><em> | ||
| + | <em><strong>Source: RCSB protein database entries <a href="http://www.rcsb.org/pdb/explore/explore.do?structureId=2WIQ">2WIQ</a> and <a href="http://www.rcsb.org/pdb/explore/explore.do?structureId=2VAD">2VAD</a>.</strong><em></p><br> | ||
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KillerRed is a 240 amino acid protein with a 3D structure similar to other fluorescent proteins, with an eleven-strand beta-barrel surrounding an alpha-helix containing the chromophore, source of the protein's optical properties.<br> | KillerRed is a 240 amino acid protein with a 3D structure similar to other fluorescent proteins, with an eleven-strand beta-barrel surrounding an alpha-helix containing the chromophore, source of the protein's optical properties.<br> | ||
KillerRed has a DsRed-type chromophore formed with residues 67Q (glutamine), 68Y (tyrosine), and 69G (glycine), to make QYG. The corresponding coding sequence can be found at the code segment CAGTACGGC.<br><br> | KillerRed has a DsRed-type chromophore formed with residues 67Q (glutamine), 68Y (tyrosine), and 69G (glycine), to make QYG. The corresponding coding sequence can be found at the code segment CAGTACGGC.<br><br> | ||
Revision as of 15:04, 19 September 2013
