Team:Groningen/Labwork/20 September 2013

From 2013.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 27: Line 27:
<br>The samples were checked on agarose gel 0.8%.
<br>The samples were checked on agarose gel 0.8%.
<br><img src="https://static.igem.org/mediawiki/igem.org/f/f8/ColonyPCR-BB_14-BB_18.jpg" width="50%">
<br><img src="https://static.igem.org/mediawiki/igem.org/f/f8/ColonyPCR-BB_14-BB_18.jpg" width="50%">
-
<br>Colony 13 from plate BBa_K1085014-BBa_K1085018 showed to be a positive candidate and was inoculated in LB + Amp.
+
<br>Colony 13 from plate BBa_K1085014-BBa_K1085018 showed to be a positive candidate (expected band ~2400 bps) and was inoculated in LB + Amp (<b>Sander</b>).
</div>
</div>

Latest revision as of 13:22, 20 September 2013

Claudio

The plate containing ligation product BBa_K1085014-BBa_K1085018 showed around a hundreds of colonies.
Colony PCR (NEB Master Mix) was performed on 16 colonies using F-hy_spank-NheI and HM14 primers (annealing temperature 70°C).
The samples were checked on agarose gel 0.8%.

Colony 13 from plate BBa_K1085014-BBa_K1085018 showed to be a positive candidate (expected band ~2400 bps) and was inoculated in LB + Amp (Sander).