Team:IIT Delhi/Safety

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style="vertical-align: top; text-align: center; background-color: rgb(51, 51, 51); width: 639px;"><big><big><big
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       <big><big><big
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style="font-family: Palatino Linotype;"><br
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      </span><font style="font-family: Palatino Linotype;"
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style="color: white;">
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size="+1"><span style="color: white;">pH
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      <font size="+2"><span style="color: white;">pH
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induced response elicited by certain promoters in bacteria may have
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induced response
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major practical applications. The response can be targeted for specific
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elicited by certain promoters in bacteria may have major practical
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pH ranges, for example in tracking the anomalies associated with the
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applications. The response can be targeted for specific pH ranges, for
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gut micro-biota or detecting pH inside a bioreactor. There are only
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example in tracking the anomalies associated with the gut micro-biota
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limited studies reported in the area. In the present project, a genetic
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or detecting pH inside a bioreactor. There are only limited studies
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circuit has been created, using the promoters of the acid shock
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reported in the area. In the present project, a genetic circuit has
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response gene from E.coli and the F0F1 ATPase operon from C. glutamicum
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been created, using the promoters of the acid shock response gene from
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that produces a pH dependent colour gradient, much like a universal pH
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E.coli and the F0F1 ATPase operon from C. glutamicum that produces a pH
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indicator. A mathematical model has been developed to simulate the
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dependent colour gradient, much like a universal pH indicator. A
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experimental findings. The present study will form the basis for
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mathematical model has been developed to simulate the experimental
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further research in the field of synthetic biology.</span></font><span
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findings. The present study will form the basis for further research in
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the field of synthetic biology.</span></font></big></big></big></td>
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style="color: white;"></span></font></span></font></td>
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  style="vertical-align: top; text-align: center; width: 49px; background-color: rgb(51, 51, 51);"></td>
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      <font style="color: white;" size="+1"><span
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style="font-family: Palatino Linotype;">For more, go to:
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igemiidelhi.org. | </span><span
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style="text-decoration: underline; font-family: Palatino Linotype;">iGEM
 +
IIT Delhi</span><span style="font-family: Palatino Linotype;">
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| Contact us at igemiitdelhi2013 at gmail dot com</span></font><br>
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Revision as of 13:38, 20 September 2013

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iGEM IIT Delhi


Project pH. Coli





Abstract

pH induced response elicited by certain promoters in bacteria may have major practical applications. The response can be targeted for specific pH ranges, for example in tracking the anomalies associated with the gut micro-biota or detecting pH inside a bioreactor. There are only limited studies reported in the area. In the present project, a genetic circuit has been created, using the promoters of the acid shock response gene from E.coli and the F0F1 ATPase operon from C. glutamicum that produces a pH dependent colour gradient, much like a universal pH indicator. A mathematical model has been developed to simulate the experimental findings. The present study will form the basis for further research in the field of synthetic biology.
















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