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Revision as of 20:30, 20 September 2013 (view source) Amberb6 (Talk | contribs) ← Older edit		Revision as of 20:53, 20 September 2013 (view source) Amberb6 (Talk | contribs) Newer edit →
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	'''IV) Actual Procedure'''		'''IV) Actual Procedure'''
-	: Gave Prapared phage to TEM operator.	+	: Gave prepared phage to TEM operator. We had four sample types:
		+	:Phage from the 'petite' band in the CsCl gradient
		+	:Phage from the 'regular/wildtype' band in the CsCl gradient
		+	:Phage from the 'mutant' band in the CsCl gradient
		+	:Phage that had 2x mutagen
	'''V) Results'''		'''V) Results'''
	:Pictures of Phage		:Pictures of Phage
		+	:Petite Phage
	[[File:Phage31_1.jpg | thumb|none|alt=]]		[[File:Phage31_1.jpg | thumb|none|alt=]]
		+	[[File:Phage31_2.jpg | thumb|none|alt=]].
		+	[[File:Phage31_3.jpg | thumb|none|alt=]]
		+	[[File:Phage31_4.jpg | thumb|none|alt=]]
		+	[[File:Phage31_5.jpg | thumb|none|alt=]]
		+	[[File:Phage31_6.jpg | thumb|none|alt=]]
		+	[[File:Phage31_7.jpg | thumb|none|alt=]]
		+	:Wild Type Phage
		+	[[File:Phage31_wt1.jpg | thumb|none|alt=]]
		+	[[File:Phage31_wt2.jpg | thumb|none|alt=]]
		+	[[File:Phage31_wt3.jpg | thumb|none|alt=]]
		+	[[File:Phage31_wt4.jpg | thumb|none|alt=]]
		+	[[File:Phage31_wt5.jpg | thumb|none|alt=]]
		+	[[File:Phage31_wt6.jpg | thumb|none|alt=]]
		+	[[File:Phage31_wt7.jpg | thumb|none|alt=]]
		+	[[File:Phage31_wt8.jpg | thumb|none|alt=]]
		+	:Mutated Phage
		+	[[File:Phage31_m1.jpg | thumb|none|alt=]]
		+	[[File:Phage31_m2.jpg | thumb|none|alt=]]
		+	[[File:Phage31_m3.jpg | thumb|none|alt=]]
		+	[[File:Phage31_m4.jpg | thumb|none|alt=]]
		+	[[File:Phage31_m5.jpg | thumb|none|alt=]]
		+	[[File:Phage31_m6.jpg | thumb|none|alt=]]
		+	:Mutated Phage - 2x Mutagen
		+	[[File:Phage31_m21.jpg | thumb|none|alt=]]
		+	[[File:Phage31_m22.jpg | thumb|none|alt=]]
		+	[[File:Phage31_m23.jpg | thumb|none|alt=]]
		+	[[File:Phage31_m24.jpg | thumb|none|alt=]]
		+	[[File:Phage31_m25.jpg | thumb|none|alt=]]
		+	[[File:Phage31_m26.jpg | thumb|none|alt=]]
	</font>		</font>

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Revision as of 20:53, 20 September 2013

• Home
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• iGEM 2013

Phage Purification July - August Notebook: Experiments

Overview March-April May-June July-August September-October

7.31 EM Pictures I) Purpose To see if we have mutated, pure phage from the CsCl gradient and mutagenisis II) Expected Outcome Pictures of pure, intact phage, potentially mutated phage. III) Reagants Used Prepared Phage IV) Actual Procedure Gave prepared phage to TEM operator. We had four sample types: Phage from the 'petite' band in the CsCl gradient Phage from the 'regular/wildtype' band in the CsCl gradient Phage from the 'mutant' band in the CsCl gradient Phage that had 2x mutagen V) Results Pictures of Phage Petite Phage . Wild Type Phage Mutated Phage File:Phage31 m1.jpg File:Phage31 m2.jpg File:Phage31 m3.jpg File:Phage31 m4.jpg File:Phage31 m5.jpg File:Phage31 m6.jpg Mutated Phage - 2x Mutagen File:Phage31 m21.jpg File:Phage31 m22.jpg File:Phage31 m23.jpg File:Phage31 m24.jpg File:Phage31 m25.jpg File:Phage31 m26.jpg