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Template:Kyoto/Notebook/Aug 28
From 2013.igem.org
(Difference between revisions)
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===Ligation=== | ===Ligation=== | ||
| + | <div class="experiment"> | ||
| + | <span class="author">No name</span> | ||
| + | {| class="wikitable" | ||
| + | !state||colspan="2"|Vector||colspan="2"|Inserter | ||
| + | |- | ||
| + | |experiment||8/71 DT-1||3.0||8/20 RBS-lysis3-1|8.0 | ||
| + | |} | ||
| + | *Samples were evaporeted used evaporator into about 3 µL. | ||
| + | {| class="wikitable" | ||
| + | !sample||MilliQ||Ligation High||total | ||
| + | |- | ||
| + | |3||4||3.5||10.5 | ||
| + | |} | ||
| + | *incubate overnight at 4 °C | ||
| + | </div> | ||
| + | |||
===Gel Extraction=== | ===Gel Extraction=== | ||
===Colony PCR=== | ===Colony PCR=== | ||
Revision as of 05:56, 21 September 2013
Contents |
Aug 28
Electrophoresis
Miniprep
| DNA | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| 8/27 Plux | 173.8 | 1.95 | 1.45 |
Ligation
| state | Vector | Inserter | ||
|---|---|---|---|---|
| experiment | 8/71 DT-1 | 3.0 | 8.0 | |
- Samples were evaporeted used evaporator into about 3 µL.
| sample | MilliQ | Ligation High | total |
|---|---|---|---|
| 3 | 4 | 3.5 | 10.5 |
- incubate overnight at 4 °C
