Template:Kyoto/Notebook/Aug 28

From 2013.igem.org

(Difference between revisions)
(→Gel Extraction: （editor:kojima）)
(→Colony PCR: editor:kojima)
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===Colony PCR===
===Colony PCR===
 +
<div class="experiment">
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<span class="author">No name</span>
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{| class="wikitable"
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!|Sample||base pair
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|-
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|8/21 RBS-lysis2-(3)||772
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|-
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|8/21 RBS-lysis2-(4)||772
 +
|-
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|8/21 RBS-lysis2-(5)||772
 +
|-
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|8/21 RBS-lysis2-(6)||772
 +
|}
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{| class="wikitable"
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!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
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|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
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|-
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|5min||30s||30s||48s||30cycles
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|}
 +
[[File:igku_Aug19electrophoresis1.png]]
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</div>
 +
===Restriction Enzyme Digestion===
===Restriction Enzyme Digestion===
===Electrophoresis===
===Electrophoresis===

Revision as of 06:14, 21 September 2013

Contents

Aug 28

Electrophoresis

No name

LaneSample
1100bp
2SasA
3RpaA
4RpaB
5PkaiBC
6PCR NC
7100bp

File:Igku xxxxxx.xxx

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
8/27 Plux173.81.951.45

Ligation

No name

stateVectorInserter
experiment8/71 DT-13.08.0
  • Samples were evaporeted used evaporator into about 3 µL.
sampleMilliQLigation Hightotal
343.510.5
  • incubate one hour at 16 °C

Gel Extraction

No name

LaneDNA
1100bpladder--
3SasA(8/27 Genome PCR production)
5RpaA(8/27 Genome PCR production)
7RpaB(8/27 Genome PCR production)
9PkaiBC(8/27 Genome PCR production)

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Colony PCR

No name

Samplebase pair
8/21 RBS-lysis2-(3)772
8/21 RBS-lysis2-(4)772
8/21 RBS-lysis2-(5)772
8/21 RBS-lysis2-(6)772
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s48s30cycles

File:Igku Aug19electrophoresis1.png

Restriction Enzyme Digestion

Electrophoresis

Gel Extraction

Colony PCR

Liquid Culture

Ligation

===Liquid Culture===
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