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Template:Kyoto/Notebook/Aug 29
From 2013.igem.org
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===Liquid Culture=== | ===Liquid Culture=== | ||
===Restriction Enzyme Digestion=== | ===Restriction Enzyme Digestion=== | ||
| + | ===Transformation=== | ||
| + | ===M9 Medium Plate=== | ||
Revision as of 09:28, 21 September 2013
Contents |
Aug 29
Colony PCR
| Sample | base pair |
|---|---|
| 8/27 Pcon-pT181 antisense-(1) | 542 |
| 8/26 pT181 attenuator-DT-(1) | 738 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 94°C | 55°C | 68°C | -- |
| 5min | 30s | 30s | 30s | 30cycles |
Electrophoresis
| Lane | Sample |
|---|---|
| 1 | 100bp ladder |
| 2 | 8/21 RBS-lysis2-9(Colony PCR prodution) |
| 3 | 8/21 RBS-lysis2-8(Colony PCR prodution) |
| 4 | 8/21 RBS-lysis2-7(Colony PCR prodution) |
| 5 | 8/21 RBS-lysis2-10(Colony PCR prodution) |
| 6 | 8/21 RBS-lysis2-11(Colony PCR prodution) |
| 7 | 8/21 RBS-lysis2-12(Colony PCR prodution) |
| 8 | 8/21 RBS-lysis2-13(Colony PCR prodution) |
| 9 | 8/21 RBS-lysis2-14(Colony PCR prodution) |
