Template:Kyoto/Notebook/Aug 29

From 2013.igem.org

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===Liquid Culture===
===Liquid Culture===
 +
<div class="experiment">
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<span class="author">No name</span>
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{| class="wikitable"
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!Sample||medium
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|-
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|8/21 RBS-Lysis2-9||Plusgrow medium(+Amp)
 +
|-
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|RBS-Lysis2-12||Plusgrow medium(+Amp)
 +
|-
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|RBS-Lysis2-14||Plusgrow medium(+Amp)　
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|}
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</div>
 +
===Restriction Enzyme Digestion===
===Restriction Enzyme Digestion===
===Transformation===
===Transformation===
===M9 Medium Plate===
===M9 Medium Plate===

Revision as of 10:08, 21 September 2013

Contents

Aug 29

Colony PCR

No name

Samplebase pair
8/27 Pcon-pT181 antisense-(1)542
8/26 pT181 attenuator-DT-(1)738
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s30s30cycles

File:Igku Aug19electrophoresis1.png

Electrophoresis

No name

LaneSample
1100bp ladder
28/21 RBS-lysis2-9(Colony PCR prodution)
38/21 RBS-lysis2-8(Colony PCR prodution)
48/21 RBS-lysis2-7(Colony PCR prodution)
58/21 RBS-lysis2-10(Colony PCR prodution)
68/21 RBS-lysis2-11(Colony PCR prodution)
78/21 RBS-lysis2-12(Colony PCR prodution)
88/21 RBS-lysis2-13(Colony PCR prodution)
98/21 RBS-lysis2-14(Colony PCR prodution)

File:Igku xxxxxx.xxx

miniprep

No Name

DNAconcentration [µg/mL]260/280260/230
Plac153.72.032.10
Pλ-luxⅠ-(1)76.52.072.06
Pλ-luxⅠ-(2)86.42.082.10
DT166.21.652.12
J23100190.11.682.34

Liquid Culture

No name

Samplemedium
8/21 RBS-Lysis2-9Plusgrow medium(+Amp)
RBS-Lysis2-12Plusgrow medium(+Amp)
RBS-Lysis2-14Plusgrow medium(+Amp)　

Restriction Enzyme Digestion

Transformation

M9 Medium Plate

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