Team:UNIK Copenhagen/Signe Notebook

From 2013.igem.org

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<div class="left_page">
<div class="left_page">
<h1>Week 1 - July 8<sup>th</sup>-14<sup>th</sup> </h1>
<h1>Week 1 - July 8<sup>th</sup>-14<sup>th</sup> </h1>
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<p> 8<sup>th</sup>-14<sup>th</sup> of July </p>
 
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<p>
<u>Culturing of magnetotactic bacteria<sup>o</sup> (MTBs)</u> <br>
<u>Culturing of magnetotactic bacteria<sup>o</sup> (MTBs)</u> <br>
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Plates were kept in a  closed container with an Oxoid AnaeroGen pad.<br><br>
Plates were kept in a  closed container with an Oxoid AnaeroGen pad.<br><br>
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<b>Assembly of constructs</b> <br>
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<u>Assembly of constructs</u> <br>
Glycerol stock of E. coli with pBBR1MCS-2 was thawed to grow on these plates.
Glycerol stock of E. coli with pBBR1MCS-2 was thawed to grow on these plates.
Primers for MamC (MS-1 strain) was ordered.  
Primers for MamC (MS-1 strain) was ordered.  

Revision as of 19:21, 21 September 2013

Notebook

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Week 1 - July 8th-14th

Culturing of magnetotactic bacteriao (MTBs)
Making base media for growing magnetotactic bacteria. This media was also used to make agar plates. MS-1 inoculated in liquid base medium. Falcon tubes were flushed with the nitrogen before use and incubated at 28 degrees. Plates were kept in a closed container with an Oxoid AnaeroGen pad.

Assembly of constructs
Glycerol stock of E. coli with pBBR1MCS-2 was thawed to grow on these plates. Primers for MamC (MS-1 strain) was ordered.

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Week 2

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