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Template:Kyoto/Notebook/Aug 31
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(Difference between revisions)
(Created page with "==Aug 31== ===Gel Extraction=== <div class="experiment"> <span class="author">No name</span> {| class="wikitable" !Lane||DNA||Enzyme |- |1||100bp ladder||-- |- |2||--||-- |- |3||...") |
(→Aug 31) |
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| Line 72: | Line 72: | ||
!Name||concentration[µg/mL]||260/280||260/230 | !Name||concentration[µg/mL]||260/280||260/230 | ||
|- | |- | ||
| - | | | + | |RBS-lysis2(EcoRI&SpeI)||4.0||2.69||0.24 |
|- | |- | ||
| - | | | + | |pSB1C3(EcoRI&SpeI)||6.0||2.48||0.05 |
|- | |- | ||
| - | | | + | |pSB1C3(XbaI&PstI)||--||--||-- |
|- | |- | ||
| - | | | + | |tRNA-Spinach||--||--||-- |
|- | |- | ||
| - | | | + | |pT181 attenuator-(2)(EcoRI&SpeI)||--||--||-- |
| + | |- | ||
| + | |pT181 anttenuator-(2)(SbaI&PstI)||--||--||-- | ||
| + | |- | ||
| + | |pT181 antisense-(2)(EcoRI&SpeI)||--||--||-- | ||
| + | |- | ||
| + | |pT181 antisense-(2)(XbaI&PstI)||--||--||-- | ||
| + | |- | ||
| + | |tetR aptamer 12_1R(EcoRI&SpeI)||--||--||-- | ||
|} | |} | ||
</div> | </div> | ||
Revision as of 13:04, 22 September 2013
Aug 31
Gel Extraction
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | -- | -- |
| 3 | RBS-lysis2-(9) | EcoRI & SpeI |
| 4 | ||
| 5 | -- | -- |
| 6 | pSB1C3(1) | EcoRI & SpeI |
| 7 | ||
| 8 | -- | -- |
| 9 | pSB1C3(1) | XbaI&PstI |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | tRNA-Spinach | EcoRI & SpeI |
| 3 | ||
| 4 | -- | -- |
| 5 | pT181 attenuator-(2) | EcoRI & SpeI |
| 6 | ||
| 7 | pT181 attenuator-(2) | XbaI & PstI |
| 8 | ||
| 9 | -- | -- |
| 10 | pT181 antisense-(2) | EcoRI & SpeI |
| 11 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | pT181 antisense | XbaRI & PstI |
| 3 | ||
| 4 | -- | -- |
| 5 | tetR aptamer 12_1R-(1) | EcoRI & SpeI |
| 6 |
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| RBS-lysis2(EcoRI&SpeI) | 4.0 | 2.69 | 0.24 |
| pSB1C3(EcoRI&SpeI) | 6.0 | 2.48 | 0.05 |
| pSB1C3(XbaI&PstI) | -- | -- | -- |
| tRNA-Spinach | -- | -- | -- |
| pT181 attenuator-(2)(EcoRI&SpeI) | -- | -- | -- |
| pT181 anttenuator-(2)(SbaI&PstI) | -- | -- | -- |
| pT181 antisense-(2)(EcoRI&SpeI) | -- | -- | -- |
| pT181 antisense-(2)(XbaI&PstI) | -- | -- | -- |
| tetR aptamer 12_1R(EcoRI&SpeI) | -- | -- | -- |
