"

From 2013.igem.org

Revision as of 16:37, 22 September 2013 (view source) Mwestb1 (Talk | contribs) m ← Older edit		Latest revision as of 18:24, 22 September 2013 (view source) Rachit (Talk | contribs) (→Protocols)
(4 intermediate revisions not shown)
Line 2:		Line 2:
	= Protocols =		= Protocols =
		+
		+	1. Plasmid extraction: ZYMO RESEARCH
		+	[http://www.zymoresearch.com/media/downloads/47/D4036i.pdf]
		+
		+	2. PCR: KOD/ Phusion Polymerase (EMD Chemicals Inc.,NJ) (Novagen Protocol)/ NEB
		+	Manufacturer’s kit
		+
		+	3. Restriction Digestion: New England Biolabs (Beverly, MA)
		+	Manufacturer’s kit
		+
		+	4. Ligation: Rapid DNA Ligase (Roche Applied Science, Indianapolis, IN).
		+	Manufacturer’s kit
		+
		+	5. Chemical competent cell and Transformation (Heat shock)
		+	(Protocol developed by hands on experience)
		+
		+	6. PCR product purification kit and gel extraction kit (ZYMO RESEARCH)

---

Latest revision as of 18:24, 22 September 2013

Protocols

1. Plasmid extraction: ZYMO RESEARCH [http://www.zymoresearch.com/media/downloads/47/D4036i.pdf]

2. PCR: KOD/ Phusion Polymerase (EMD Chemicals Inc.,NJ) (Novagen Protocol)/ NEB Manufacturer’s kit

3. Restriction Digestion: New England Biolabs (Beverly, MA) Manufacturer’s kit

4. Ligation: Rapid DNA Ligase (Roche Applied Science, Indianapolis, IN). Manufacturer’s kit

5. Chemical competent cell and Transformation (Heat shock) (Protocol developed by hands on experience)

6. PCR product purification kit and gel extraction kit (ZYMO RESEARCH)