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Geraniol is an intriguing 10 carbon compound with diverse applications including use as an agent for cancer prevention, fragrance, insect repellent, proposed biofuel etc. We explored and engineered a novel gene expression tool (BBa_K890000) for ''Methanococcus'' with the capability of expressing geraniol synthase from ''Ocimum basilicum'' (BBa_K1138000). We report the biosynthesis of geraniol at over 5% of DCW by transforming the vector into Methanococcus thereby expanding its native isoprenoid pathway. Furthermore we engineered new vectors (BBa_K1138001 & BBa_K1138002) with the potential capability of regulating and quantifying the expression of desired proteins via red fluorescence. This work demonstrates the use of ''Methanococcus'' as a cell factory for chemical production and highlights synthetic biology advancement by engineering new systems over traditional biological systems such as ''Escherichia coli''. | Geraniol is an intriguing 10 carbon compound with diverse applications including use as an agent for cancer prevention, fragrance, insect repellent, proposed biofuel etc. We explored and engineered a novel gene expression tool (BBa_K890000) for ''Methanococcus'' with the capability of expressing geraniol synthase from ''Ocimum basilicum'' (BBa_K1138000). We report the biosynthesis of geraniol at over 5% of DCW by transforming the vector into Methanococcus thereby expanding its native isoprenoid pathway. Furthermore we engineered new vectors (BBa_K1138001 & BBa_K1138002) with the potential capability of regulating and quantifying the expression of desired proteins via red fluorescence. This work demonstrates the use of ''Methanococcus'' as a cell factory for chemical production and highlights synthetic biology advancement by engineering new systems over traditional biological systems such as ''Escherichia coli''. | ||
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| - | + | '''Geraniol production via novel protein expression tools in ''Methanococcus maripaludis''''' | |
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| - | + | Geraniol is an intriguing 10 carbon compound with diverse applications including use as an agent for cancer prevention, fragrance, insect repellent, proposed biofuel etc. We explored and engineered a novel gene expression tool (BBa_K890000) for ''''Methanococcus'''' with the capability of expressing geraniol synthase from ''Ocimum basilicum'' (BBa_K1138000). We report the biosynthesis of geraniol at over 5% of DCW by transforming the vector into Methanococcus thereby expanding its native isoprenoid pathway. Furthermore we engineered new vectors (BBa_K1138001 & BBa_K1138002) with the potential capability of regulating and quantifying the expression of desired proteins via red fluorescence. This work demonstrates the use of ''Methanococcus'' as a cell factory for chemical production and highlights synthetic biology advancement by engineering new systems over traditional biological systems such as ''Escherichia coli''. | |
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Revision as of 18:48, 22 September 2013
Welcome to the University of Georgia 2013 iGEM Team Wiki!
Contents |
Introduction
Geraniol production via novel protein expression tools in Methanococcus maripaludis
Geraniol is an intriguing 10 carbon compound with diverse applications including use as an agent for cancer prevention, fragrance, insect repellent, proposed biofuel etc. We explored and engineered a novel gene expression tool (BBa_K890000) for Methanococcus with the capability of expressing geraniol synthase from Ocimum basilicum (BBa_K1138000). We report the biosynthesis of geraniol at over 5% of DCW by transforming the vector into Methanococcus thereby expanding its native isoprenoid pathway. Furthermore we engineered new vectors (BBa_K1138001 & BBa_K1138002) with the potential capability of regulating and quantifying the expression of desired proteins via red fluorescence. This work demonstrates the use of Methanococcus as a cell factory for chemical production and highlights synthetic biology advancement by engineering new systems over traditional biological systems such as Escherichia coli.
Abstract
Geraniol production via novel protein expression tools in Methanococcus maripaludis
Geraniol is an intriguing 10 carbon compound with diverse applications including use as an agent for cancer prevention, fragrance, insect repellent, proposed biofuel etc. We explored and engineered a novel gene expression tool (BBa_K890000) for 'Methanococcus' with the capability of expressing geraniol synthase from Ocimum basilicum (BBa_K1138000). We report the biosynthesis of geraniol at over 5% of DCW by transforming the vector into Methanococcus thereby expanding its native isoprenoid pathway. Furthermore we engineered new vectors (BBa_K1138001 & BBa_K1138002) with the potential capability of regulating and quantifying the expression of desired proteins via red fluorescence. This work demonstrates the use of Methanococcus as a cell factory for chemical production and highlights synthetic biology advancement by engineering new systems over traditional biological systems such as Escherichia coli.
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