Team:Glendale CC AZ/Protocols/SurivalGrowth
From 2013.igem.org
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1. Label all plates (Keep upside down to avoid condensation on agar) | 1. Label all plates (Keep upside down to avoid condensation on agar) |
Revision as of 02:24, 23 September 2013
Glendale Community College Arizona
Protocols
Survival Growth Assay
Materials
-P200 micropipette -10µl bacteria -90µl LB media -Agar plates with appropriate antibiotic -Spreader -20mL ethanol (to sterilize spreader) -Incubator set to 37°C.
Procedure
1. Label all plates (Keep upside down to avoid condensation on agar)
2. Pipette LB media into tube containing bacteria; aspirate.
3. Using P200 micropipette, extract all 100µl of solution and place onto appropriate agar plate.
4. Briefly flame the spreader and let cool momentarily.
5. Spread bacteria-LB solution all over plate.
6. Incubate at 37°C for up to 24 hours.