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| | Use this page to answer the questions on the [[Safety | safety page]]. | | Use this page to answer the questions on the [[Safety | safety page]]. |
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| - | iGEM Safety Questions
| + | '''Safety forms were approved on September 22, 2013 by Evan Appleton.''' |
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| - | '''<u>1. Would any of your project ideas raise safety issues in term of:</u>'''
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| - | '''-researcher safety'''
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| - | We are using the bacteria Burkholderia xenovorans, Pseudomonas pseudoalcaligenes KF707,
| + | iGEM Safety Questions |
| - | Rhodococcus jostii RHA1 and Escherichia coli DH5 a to build the biobrick parts, and
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| - | especially Escherichia coli DH5 a as the host of our constructs. These strains are not pathogenic
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| - | so they belong to the risk group 1 according to the WHO laboratory biosafety manual.
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| - | These organisms have been manipulated with all the required precautions without any risk or
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| - | danger for our researchers leading the experiments.
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| - | Nevertheless, for our project a chemical pollutant the PCB (Polychlorinated Biphenyl) has
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| - | been employed so as to test the efficiency of our constructs. This hydrophobic molecule can
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| - | penetrate skin and latex, it is able to induct cardiovascular disease and maybe cancer, that is
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| - | why we manipulated it with the material and methods introduced by the IOMC (Inter-Organization
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| - | Program for the Sound Management of Chemicals) letting us manipulate it safely.
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| - | http://www.chem.unep.ch/pops/pdf/pcbtranscap.pdf
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| - | '''-public safety'''
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| - | During the course of an experiment, we made sure members of the general public did not have
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| - | access to the lab by keeping the door locked. The lack of pathogenic power of these strains
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| - | avoided all danger of illness diffusion by an unlikely exposition.
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| - | Concerning PCBs, their manipulation was done in confined conditions where anybody
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| - | unexpected could be in contact with this pollutant.
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| - | '''-environmental safety'''
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| - | Our project has been made to be applied in the case of a confined environment where
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| - | microorganisms won’t vanish within the air steering clear any DNA transfer with another
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| - | form of life. If we expect the worst these genes would confer the ability of catalyze the degradation
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| - | or detect PCBs what do not represent a real danger for environment if we do not care
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| - | about the probability of mutations modifying their function or quite simply the ethical limits
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| - | of GMOs released in nature.
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| - | '''<u>2.Do any of the BioBrick parts (or devices) that you made this year raise safety issues?</u>'''
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| - | Not really, this year the biobricks will concern the detection of PCBs and the coordination of
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| - | the biodegradation metabolism of those in E.Coli, the parts are not dangerous as they are.
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| | + | '''1. Please describe the chassis organism(s) you will be using for this project. If you will be using more than one chassis organism, provide information on each of them:''' |
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| | + | We used the strain ''E. coli'' K12, the MG1655 strain and its derivatives, which all belong to Risk group 1 ([http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli]) and is not associated with disease in healthy adult humans. |
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| - | '''<u>3.Is there a local biosafety group, committee, or review board at your institution?</u>'''
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| - | There is, in every department of the Université Paris-Sud, a comity in charge of hygiene and
| + | '''2. Highest Risk Group Listed:''' |
| - | security. Consequently, the Institute of Genetics and Microbiology, where we performed our
| + | |
| - | experiments, has a bureau in charge of hygiene and security whose GMO section is directed by one
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| - | of our instructors: Jean-Luc Pernodet.
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| - | His presence in the team helped us think of the dangers linked to disseminating GMOs into the
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| - | environment, as well as ways to resolve these problems.
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| - | We also learned about the specific safety rules of the laboratory where we carried our experiments
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| - | with the person in charge of this laboratory (gas usage, handling of BEt (ethidium bromide),
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| - | processing of chemical and biological waste...).
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| - | We also payed attention to the security memorandum and signed the hygiene and security
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| - | charter of the Institute of Genetics and Microbiology:
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| - | Security memorandum
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| - | Hygiene and security charter
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| - | All experiments were carried out according to French Safety Regulations.
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| | + | The highest risk group listed is 1. |
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| - | '''<u>4. Do you have any other ideas how to deal with safety issues that could be useful for | + | '''3. List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013 iGEM Distribution without modifying them, you do not need to list those parts.)''' |
| - | future iGEM competitions? How parts devices and systems could be made even safer
| + | |
| - | through biosafety engineering</u>'''
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| - | The advocation by the iGEM competition of the restriction enzymes and particularly the 3A
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| - | assembly, even if we are fully aware of the fact that it’s a way to reach compatibility, could be, in
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| - | terms of biosafety, a mean to increase the probability of DNA transfer between organisms because
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| - | of the excessive use of restriction sites, in the prefix and the suffix, recognized by enzymes that can
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| - | be easily found, in high levels, in most of organisms.
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| - | Some ways using methods like Gibson Assembly should be more frequently employed to make the
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| - | constructs, even if the biobrick standard would be harder to approach.
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| - | However the risk of DNA transformation by homologous sequences remains in spite of the
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| - | reduction of biohazard. Obviously it’s only a hypothesis whose hasn’t been checke
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| | {{Team:Paris_Saclay/incl_fin}} | | {{Team:Paris_Saclay/incl_fin}} |
Safety
Use this page to answer the questions on the safety page.
Safety forms were approved on September 22, 2013 by Evan Appleton.
iGEM Safety Questions
1. Please describe the chassis organism(s) you will be using for this project. If you will be using more than one chassis organism, provide information on each of them:
We used the strain E. coli K12, the MG1655 strain and its derivatives, which all belong to Risk group 1 ([http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli]) and is not associated with disease in healthy adult humans.
2. Highest Risk Group Listed:
The highest risk group listed is 1.
3. List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013 iGEM Distribution without modifying them, you do not need to list those parts.)
"
