Team:Groningen/Labwork/15 September 2013

From 2013.igem.org

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<h2>Claudio</h2>
<h2>Claudio</h2>
Plates containing pSB1C3-S1-S5-S5-S13 and pSB1C3-S2-S5-S5-S14 showed thousands of colonies.
Plates containing pSB1C3-S1-S5-S5-S13 and pSB1C3-S2-S5-S5-S14 showed thousands of colonies.
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<br>Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65&deg;C).
 
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<br>Plates containing pSB1C3-S16-S3-S5-S13 and pSB1C3-S16-S9-S11-S14 showed thousands of colonies.
 
<br>Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65&deg;C).
<br>Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65&deg;C).
<br>The samples were checked on agarose gel 0.8%.
<br>The samples were checked on agarose gel 0.8%.
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<br><img src="" width="50%">
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<br><img src="https://static.igem.org/mediawiki/2013/0/01/ColonyPCR_double5_up_2013-09-15_14hr_38min.tif" width="60%">
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<br>Colony ?? from ?? and colony ?? from ?? show to be positive candidates and were inoculated in LB + Cm.
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<br>Colony C from pSB1C3-S1-S5-S5-S13 and colony A from pSB1C3-S2-S5-S5-S14 showed to be positive candidates and were inoculated in LB + Cm.
<br>
<br>
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<br>Colony PCR (NEB Master Mix) was performed on 4 colonies from plate pSB1C3-S2-S5-S14 using VF2 and VR primers (annealing temperature 65&deg;C).
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<br>Plates containing pSB1C3-S16-S3-S5-S13 and pSB1C3-S16-S9-S11-S14 showed thousands of colonies.
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<br>Colony PCR (NEB Master Mix) was performed on 4 colonies from plate BBa_K1085014-S1-S5-S14 using F_spank_NheI and HM14 primers (annealing temperature 68&deg;C).
+
<br>Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65&deg;C).
<br>The samples were checked on agarose gel 0.8%.
<br>The samples were checked on agarose gel 0.8%.
-
<br><img src="" width="50%">
+
<br>The gel didn't run well, however, one colony per plate was inoculated in LB + Cm for further screening.
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<br>Colony ?? from plate ?? showed a band at the expected height (~2400 bps). Therefore was inoculated in LB + Cm.
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<Br>
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<br>Colony ?? from plate ?? showed a band at the expected height (~2400 bps). Therefore was inoculated in LB + Cm.
+
<Br>
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<h2>Sebas</h2>
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All four colonies were red on plate.
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<br>Inoculated 1 colony (#16) twice in 3ml LB/amp.  
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<Br>
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Latest revision as of 09:15, 23 September 2013

Claudio

Plates containing pSB1C3-S1-S5-S5-S13 and pSB1C3-S2-S5-S5-S14 showed thousands of colonies.
Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65°C).
The samples were checked on agarose gel 0.8%.

Colony C from pSB1C3-S1-S5-S5-S13 and colony A from pSB1C3-S2-S5-S5-S14 showed to be positive candidates and were inoculated in LB + Cm.

Plates containing pSB1C3-S16-S3-S5-S13 and pSB1C3-S16-S9-S11-S14 showed thousands of colonies.
Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65°C).
The samples were checked on agarose gel 0.8%.
The gel didn't run well, however, one colony per plate was inoculated in LB + Cm for further screening.

Sebas

All four colonies were red on plate.
Inoculated 1 colony (#16) twice in 3ml LB/amp.