Template:Kyoto/Notebook/Sep 4

From 2013.igem.org

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(→Sep 4)
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===Colony PCR===
===Colony PCR===
<div class="experiment">
<div class="experiment">
-
<span class="author">Kojima</span>
+
<span class="author">Hirano</span>
-
{| class="wikitable"
+
-
!|Sample||base pair
+
-
|-
+
-
|8/18 J23100 control -(1)||1142
+
-
|-
+
-
|8/18 J23100-RBS-GFP-DT -(1)||1156
+
-
|-
+
-
|8/18 J23100-RBS-GFP-DT -(2)||1156
+
-
|-
+
-
|8/18 J23100-RBS-luxR-DT -(1)||1271
+
-
|-
+
-
|8/18 J23100-RBS-luxR-DT -(2)||1271
+
-
|-
+
-
|8/18 J23100-RBS-lacZ&alpha;-DT -(1)||670
+
-
|-
+
-
|8/18 J23100-RBS-lacZ&alpha;-DT -(2)||670
+
-
|-
+
-
|8/18 RBS-lysis3 -(1)||997
+
-
|-
+
-
|8/18 RBS-lysis3 -(2)||997
+
-
|-
+
-
|8/18 P0440(RBS-tetR-DT) -(1)||1154
+
-
|-
+
-
|8/18 P0440(RBS-tetR-DT) -(2)||1154
+
-
|-
+
-
|negative control||--
+
-
|}
+
{| class="wikitable"
{| class="wikitable"
!PreDenature||Denature||Annealing||Extension||cycle
!PreDenature||Denature||Annealing||Extension||cycle

Revision as of 12:20, 23 September 2013

Contents

Sep 4

Gel Extraction

No name

1

LaneDNAEnzyme
1100bp ladder--
2PconSpe1&Pst1
3PconSpe1&Pst1
4----
5PlacSpe1&Pst1
6PlacSpe1&Pst1

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

</div>

No name

2

LaneDNAEnzyme
1100bp ladder--
2PtetSpe1&Pst1
3PtetSpe1&Pst1
4----
5RBS-tetR-DTXba1&Pst1
6RBS-tetR-DTXba1&Pst1

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Master Plate

No name

NumberUse LB plate(+Amp)
48/9 BBa-J23100-3
58/9 BBa-J23100-4
68/9 BBa-J23100-5

Master Plate

Hirano

NumberUse LB plate
1JM109-1
2JM109-2
3JM109-3
4JM109-4


Liquid Culture

Hirano

Samplemedium
8/9 BBa-J23100-3Plusgrow medium(+Amp)

Liquid Culture

Hirano

Samplemedium
JM109-1(Master plate)LB medium

Colony PCR

Hirano

PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30cycles

File:Igku Aug19electrophoresis1.png

Samplebase pair
8/9 BBa-J23100 3--
8/9 BBa-J23100 4--
8/9 BBa-J23100 5--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s30s30cycles

File:Igku Aug19electrophoresis2.png

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