Template:Kyoto/Notebook/Aug 17
From 2013.igem.org
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- | == | + | ===Colony PCR=== |
+ | <div class="experiment"> | ||
+ | <span class="author">Nakamoto</span> | ||
+ | {| class="wikitable" | ||
+ | !|Sample||base pair | ||
+ | |- | ||
+ | |8/15 BBa_K112806(lysis2) -(1)||828 | ||
+ | |- | ||
+ | |negative control||-- | ||
+ | |} | ||
+ | {| class="wikitable" | ||
+ | !PreDenature||Denature||Annealing||Extension||cycle | ||
+ | |- | ||
+ | |94°C||94°C||55°C||68°C||-- | ||
+ | |- | ||
+ | |5min||30s||30s||1min||30cycles | ||
+ | |} | ||
+ | <!-- [[File:image]] --> | ||
+ | {| class="wikitable" | ||
+ | !Sample||base pair | ||
+ | |- | ||
+ | |8/10 BBa_Q0400 -(1)||3995 | ||
+ | |- | ||
+ | |negative control||-- | ||
+ | |} | ||
+ | {| class="wikitable" | ||
+ | !PreDenature||Denature||Annealing||Extension||cycle | ||
+ | |- | ||
+ | |94°C||94°C||55°C||68°C||-- | ||
+ | |- | ||
+ | |5min||30s||30s||30s||30cycles | ||
+ | |} | ||
+ | [[File:igku_Aug19electrophoresis2.png]] | ||
+ | </div> | ||
+ | <br> |
Revision as of 13:26, 23 September 2013
Colony PCR
Sample | base pair |
---|---|
8/15 BBa_K112806(lysis2) -(1) | 828 |
negative control | -- |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94°C | 94°C | 55°C | 68°C | -- |
5min | 30s | 30s | 1min | 30cycles |
Sample | base pair |
---|---|
8/10 BBa_Q0400 -(1) | 3995 |
negative control | -- |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94°C | 94°C | 55°C | 68°C | -- |
5min | 30s | 30s | 30s | 30cycles |